RNA Interference on Regenerating Holothurian Gut Tissues

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2020 Oct 19

PMID 33074545

Citations 5

Authors

Miosotis Alicea-Delgado

Samir A Bello-Melo

Jose E Garcia-Arraras

Affiliations

Soon will be listed here.

Abstract

Functional studies on echinoderms have been reduced to the use of pharmacological treatments. The ability to modulate the genetic expression of regenerating tissues can elucidate potential effectors during this process. Here we describe an effective transfection protocol that allows the introduction of Dicer-substrate interference RNAs (DsiRNAs) for the modulation of gene expression and its characterization during regeneration.

Citing Articles

Sea cucumbers: an emerging system in evo-devo.

Perillo M, Sepe R, Paganos P, Toscano A, Annunziata R Evodevo. 2024; 15(1):3.

PMID: 38368336 PMC: 10874539. DOI: 10.1186/s13227-023-00220-0.

Intestine Explants in Organ Culture: A Tool to Broaden the Regenerative Studies in Echinoderms.

Bello S, Garcia-Arraras J J Mar Sci Eng. 2022; 10(2).

PMID: 36284610 PMC: 9592059. DOI: 10.3390/jmse10020244.

Regeneration in Echinoderms: Molecular Advancements.

Medina-Feliciano J, Garcia-Arraras J Front Cell Dev Biol. 2022; 9:768641.

PMID: 34977019 PMC: 8718600. DOI: 10.3389/fcell.2021.768641.

Ancient role of sulfakinin/cholecystokinin-type signalling in inhibitory regulation of feeding processes revealed in an echinoderm.

Tinoco A, Barreiro-Iglesias A, Alfonso Yanez Guerra L, Delroisse J, Zhang Y, Gunner E Elife. 2021; 10.

PMID: 34488941 PMC: 8428848. DOI: 10.7554/eLife.65667.

Wnt/β-catenin signaling pathway regulates cell proliferation but not muscle dedifferentiation nor apoptosis during sea cucumber intestinal regeneration.

Alicea-Delgado M, Garcia-Arraras J Dev Biol. 2021; 480:105-113.

PMID: 34481794 PMC: 9503151. DOI: 10.1016/j.ydbio.2021.08.011.

References

Elbashir S, Lendeckel W, Tuschl T . RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 2001; 15(2):188-200. PMC: 312613. DOI: 10.1101/gad.862301. View

Bello S, Abreu-Irizarry R, Garcia-Arraras J . Primary cell cultures of regenerating holothurian tissues. Methods Mol Biol. 2014; 1189:283-97. PMC: 5426911. DOI: 10.1007/978-1-4939-1164-6_19. View

Hannon G . RNA interference. Nature. 2002; 418(6894):244-51. DOI: 10.1038/418244a. View

Fire A, Xu S, Montgomery M, Kostas S, Driver S, Mello C . Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature. 1998; 391(6669):806-11. DOI: 10.1038/35888. View

Bogarad L, Arnone M, Chang C, Davidson E . Interference with gene regulation in living sea urchin embryos: transcription factor knock out (TKO), a genetically controlled vector for blockade of specific transcription factors. Proc Natl Acad Sci U S A. 1998; 95(25):14827-32. PMC: 24534. DOI: 10.1073/pnas.95.25.14827. View