Nile-Red-Based Fluorescence Probe for Selective Detection of Biothiols, Computational Study, and Application in Cell Imaging

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Oct 17

PMID 33066675

Citations 3

Authors

Xiang Rong

Zhong-Yong Xu

Jin-Wu Yan

Zhi-Zhong Meng

Bin Zhu

Lei Zhang

Affiliations

Soon will be listed here.

Abstract

A new colorimetric and fluorescence probe based on Nile-red chromophore for the detection of biothiols has been developed, exhibiting high selectivity towards biothiols over other interfering species. shows a blue shift in absorption peak upon reacting with biothiols, from 587 nm to 567 nm, which induces an obvious color change from blue to pink and exhibits a 35-fold fluorescence enhancement at 645 nm in red emission range. displays rapid (<1 min) response for HS, which is faster than other biothiols (>5 min). The detection limits of probe towards biothiols are very low (22.05 nM for HS, 34.04 nM for Cys, 107.28 nM for GSH and 113.65 nM for Hcy). Furthermore, is low cytotoxic and can be successfully applied as a bioimaging tool for real-time monitoring biothiols in HeLa cells. In addition, fluorescence mechanism of probe is further understood by theoretical calculations.

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