Differential Analysis of Serum Principal Components Treated with Compound Decoction and Related Compounds Based on High-Resolution Mass Spectrometry (HRMS)

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2020 Oct 16

PMID 33062021

Citations 2

Authors

Wanjin Sun

Junjie Zhang

Conghui Zhou

Bin Yan

Quan Cai

Hongxia He

Xueyun Duan

Heng Fan

Affiliations

Soon will be listed here.

Abstract

Objective: To compare the differences in the serum principal components in ulcerative colitis- (UC-) induced rats, treated with compound decoction, matrine, oxymatrine monomer mixture, and indirubin monomer, and to provide a modern scientific basis for elucidating the clinical efficacy of compound decoction for the treatment of UC.

Methods: The serum samples of rats from each group were obtained after drug administration, and the serum principal components of each group were analyzed by high-resolution mass spectrometry. Agilent Eclipse XDB C18 chromatographic column (100 mm × 2.1 mm, 3.5 m) was used for separation. The mobile phase was water (A) and methanol (B) (0.1% formic acid) gradient elution, 0-3 min (B: 20%-40%), 3-10 min (B: 40%-54%), 10-25 min (B: 54%), 25-35 min (B: 54%-70%), 35-45 min (B: 70%-80%), 45-50 min (B: 80%), 50-60 min (B: 80%-100%), 70-72 min (B: 100%-20%), and 72-77 min (B: 20%); flow rate, 300 L/min; column temperature, 40°C; and injection volume, 10 L. ESI source was selected and scanned in the positive and negative ion modes. The scanning range was 70-1500 /z; ion-source gas 1 (GS1): 55 psi; ion-source gas 2 (GS2): 60 psi; CUR: 30 psi; ion-source temperature (TEM): 550°C; ion-source voltage (ISVF) : 5500 V/-4500 V; decluster voltage (DP): 100 V; collision energy (CE): 35 V/-35 V; collision energy gain (CES) : 15 V/-15 V; and data acquisition mode: IDA. After the data from each group were imported into MarkView 1.3, the molecular weights and retention times of different substances were obtained and qualitatively analyzed by ChemSpider and PeakView 2.0.

Results: In the negative ion mode, 26 differential compounds were identified in the compound decoction group (FFKST) compared to the model group (M), and 18 differential compounds were identified in the matrine and oxymatrine group (KST) compared to the model group (M). In the positive ion mode, 11 and 7 differential compounds were identified in the compound decoction group (FFKST) and the matrine and oxymatrine group (KST) compared to the model group (M), respectively. The responses of all compounds in each group were compared with each other. As the different principal component substances in the indirubin group (DYH) displayed little correlation with other groups, the different components in this group were not researched thoroughly.

Conclusion: By comparing the differences in the serum principal components from each administration group, we found that the FFKST group exhibited enhanced synthesis of the serum principal components; however, the compound doses of matrine and oxymatrine monomers did not exhibit the same changes in the serum principal components of UC-induced rats. Finally, the traditional Chinese medicine compound is more advantageous than monomers.

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