Redundant Type II Cadherins Define Neuroepithelial Cell States for Cytoarchitectonic Robustness

Overview

Journal Commun Biol

Specialty Biology

Date 2020 Oct 16

PMID 33060832

Citations 5

Authors

Kou Hiraga

Yukiko U Inoue

Junko Asami

Mayuko Hotta

Yuki Morimoto

Shoji Tatsumoto

Mikio Hoshino

Yasuhiro Go

Takayoshi Inoue

Affiliations

Soon will be listed here.

Abstract

Individual cell shape and integrity must precisely be orchestrated during morphogenesis. Here, we determine function of type II cadherins, Cdh6, Cdh8, and Cdh11, whose expression combinatorially demarcates the mouse neural plate/tube. While CRISPR/Cas9-based single type II cadherin mutants show no obvious phenotype, Cdh6/8 double knockout (DKO) mice develop intermingled forebrain/midbrain compartments as these two cadherins' expression opposes at the nascent boundary. Cdh6/8/11 triple, Cdh6/8 or Cdh8/11 DKO mice further cause exencephaly just within the cranial region where mutated cadherins' expression merges. In the Cdh8/11 DKO midbrain, we observe less-constricted apical actin meshwork, ventrally-directed spreading, and occasional hyperproliferation among dorsal neuroepithelial cells as origins for exencephaly. These results provide rigid evidence that, by conferring distinct adhesive codes to each cell, redundant type II cadherins serve essential and shared roles in compartmentalization and neurulation, both of which proceed under the robust control of the number, positioning, constriction, and fluidity of neuroepithelial cells.

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