Luminescence of Luciferin in the Presence of Human Plasma Alpha 1-Acid Glycoprotein

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Oct 15

PMID 33053850

Citations 4

Authors

Shusei Kanie

Mami Komatsu

Yasuo Mitani

Affiliations

Soon will be listed here.

Abstract

The enzyme luciferase (CLase) enables luciferin to emit light efficiently through an oxidation reaction. The catalytic mechanism on the substrate of CLase has been studied, but the details remain to be clarified. Here, we examined the luminescence of luciferin in the presence of several proteins with drug-binding ability. Luminescence measurements showed that the mixture of human plasma alpha 1-acid glycoprotein (hAGP) and luciferin produced light. The total value of the luminescence intensity over 60 s was over 12.6-fold higher than those in the presence of ovalbumin, human serum albumin, or bovine serum albumin. In the presence of heat-treated hAGP, the luminescence intensity of luciferin was lower than in the presence of intact hAGP. Chlorpromazine, which binds to hAGP, showed an inhibitory effect on the luminescence of luciferin, both in the presence of hAGP and a recombinant CLase. Furthermore, BlastP analysis showed that hAGP had partial amino acid sequence similarity to known CLases in the region including amino acid residues involved in the drug-binding ability of hAGP. These findings indicate enzymological similarity between hAGP and CLase and provide insights into both the enzymological understanding of CLase and development of a luminescence detection method for hAGP.

Citing Articles

Bioluminescence of ()-Cypridina Luciferin with Luciferase.

Kanie S, Wu C, Kihira K, Yasuno R, Mitani Y, Ohmiya Y Int J Mol Sci. 2024; 25(5).

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Pseudo-Luciferase Activity of the SARS-CoV-2 Spike Protein for Luciferin.

Nishihara R, Dokainish H, Kihara Y, Ashiba H, Sugita Y, Kurita R ACS Cent Sci. 2024; 10(2):283-290.

PMID: 38435535 PMC: 10906034. DOI: 10.1021/acscentsci.3c00887.

Potential use of Cypridina luciferin for quantifying alpha 1-acid glycoprotein in human serum.

Kanie S, Mitani Y Anal Sci. 2022; 38(12):1555-1562.

PMID: 36205879 DOI: 10.1007/s44211-022-00191-7.

Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog.

Sousa J, Magalhaes C, Gonzalez-Berdullas P, da Silva J, da Silva L Int J Mol Sci. 2022; 23(15).

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