Regulation of γδT17 Cells by Mycobacterium Vaccae Through Interference with Notch/Jagged1 Signaling Pathway

Overview

Journal Braz J Med Biol Res

Specialties Biology
General Medicine

Date 2020 Oct 14

PMID 33053115

Authors

Yi En Yao

Jing Hong Zhang

Xiao Ju Chen

Jian Lin Huang

Qi Xiang Sun

Wei Wei Liu

Huan Zeng

Chao Qian Li

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.

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