The Pathogenic Effects of on the Proliferation, Osteogenic Differentiation, and Transcriptome of Osteoblasts

Overview

Journal Front Cell Dev Biol

Specialty Cell Biology

Date 2020 Oct 12

PMID 33042984

Citations 8

Authors

Hui Gao

Tianyong Sun

Fanghong Yang

Jiakan Yuan

Mei Yang

Wenyan Kang

Di Tang

Jun Zhang

Qiang Feng

Affiliations

Soon will be listed here.

Abstract

As one of the most common oral diseases, periodontitis is closely correlated with tooth loss in middle-aged and elderly people. () contributes to periodontitis, but the evidence in alveolar bone loss is still unclear. In this study, cytological experiments and transcriptome analyses were performed to characterize the biological process abnormalities and the molecular changes of -stimulated osteoblasts. could inhibit cell proliferation, promote cell apoptosis, and elevate pro-inflammatory cytokine production of osteoblasts, and it also inhibited osteoblast differentiation and mineralized nodule formation and decreased the expression of osteogenetic genes and proteins. Whole-transcriptome analyses identified a total of 235 transcripts that were differentially expressed in all six time points, most of which were inflammation-related genes. The genes, , , , , , , , , , , , , and , played core roles in a PPI network, and interacted closely with other ones in the infection. In addition, 133 osteogenesis-related differential expression genes (DEGs) were time-serially dynamically changed in a short time-series expression miner (STEM) analysis, which were enriched in multiple cancer-related pathways. The core dynamic DEGs (, , , , , , , and ) had been reported to be closely related to the development and metastasis in tumor and cancer progress. This study is the first to evaluate the long-term interaction of on osteoblasts, which might increase the risk of cell carcinogenesis of normal osteoblasts, and provides new insight into the pathogenesis of bacterial-induced bone destruction.

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References

Kolenbrander P . Multispecies communities: interspecies interactions influence growth on saliva as sole nutritional source. Int J Oral Sci. 2011; 3(2):49-54. PMC: 3469885. DOI: 10.4248/IJOS11025. View

von Mering C, Jensen L, Snel B, Hooper S, Krupp M, Foglierini M . STRING: known and predicted protein-protein associations, integrated and transferred across organisms. Nucleic Acids Res. 2004; 33(Database issue):D433-7. PMC: 539959. DOI: 10.1093/nar/gki005. View

Lillo Osuna M, Garcia-Lopez J, El Ayachi I, Fatima I, Khalid A, Kumpati J . Activation of Estrogen Receptor Alpha by Decitabine Inhibits Osteosarcoma Growth and Metastasis. Cancer Res. 2018; 79(6):1054-1068. PMC: 6420872. DOI: 10.1158/0008-5472.CAN-18-1255. View

Hoppe T, Goser V, Kraus D, Probstmeier R, Frentzen M, Wenghoefer M . Response of MG63 osteoblasts on bacterial challenge is dependent on the state of differentiation. Mol Oral Microbiol. 2017; 33(2):133-142. DOI: 10.1111/omi.12203. View

Zhou Y, Zhou B, Pache L, Chang M, Khodabakhshi A, Tanaseichuk O . Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat Commun. 2019; 10(1):1523. PMC: 6447622. DOI: 10.1038/s41467-019-09234-6. View

Salim A, Nacamuli R, Morgan E, Giaccia A, Longaker M . Transient changes in oxygen tension inhibit osteogenic differentiation and Runx2 expression in osteoblasts. J Biol Chem. 2004; 279(38):40007-16. DOI: 10.1074/jbc.M403715200. View

Kang W, Jia Z, Tang D, Zhang Z, Gao H, He K . Facilitates Apoptosis, ROS Generation, and Inflammatory Cytokine Production by Activating AKT/MAPK and NF-B Signaling Pathways in Human Gingival Fibroblasts. Oxid Med Cell Longev. 2019; 2019:1681972. PMC: 6815639. DOI: 10.1155/2019/1681972. View

Geng F, Zhang Y, Lu Z, Zhang S, Pan Y . Caused DNA Damage and Promoted Cell Proliferation by the / Pathway in Oral Cancer Cells. DNA Cell Biol. 2019; 39(1):144-151. PMC: 6978777. DOI: 10.1089/dna.2019.5064. View

Utting J, Robins S, Brandao-Burch A, Orriss I, Behar J, Arnett T . Hypoxia inhibits the growth, differentiation and bone-forming capacity of rat osteoblasts. Exp Cell Res. 2006; 312(10):1693-702. DOI: 10.1016/j.yexcr.2006.02.007. View

10.

Park O, Kim J, Yang J, Yun C, Han S . Enterococcus faecalis Inhibits Osteoblast Differentiation and Induces Chemokine Expression. J Endod. 2015; 41(9):1480-5. DOI: 10.1016/j.joen.2015.04.025. View

11.

Kook J, Park S, Lim Y, Cho E, Jo E, Roh H . Genome-Based Reclassification of Fusobacterium nucleatum Subspecies at the Species Level. Curr Microbiol. 2017; 74(10):1137-1147. DOI: 10.1007/s00284-017-1296-9. View

12.

Yang Y, Weng W, Peng J, Hong L, Yang L, Toiyama Y . Fusobacterium nucleatum Increases Proliferation of Colorectal Cancer Cells and Tumor Development in Mice by Activating Toll-Like Receptor 4 Signaling to Nuclear Factor-κB, and Up-regulating Expression of MicroRNA-21. Gastroenterology. 2016; 152(4):851-866.e24. PMC: 5555435. DOI: 10.1053/j.gastro.2016.11.018. View

13.

Morton T, Clagett J, Yavorsky J . Role of immune complexes in human periapical periodontitis. J Endod. 1977; 3(7):261-8. DOI: 10.1016/S0099-2399(77)80045-9. View

14.

Ernst J, Nau G, Bar-Joseph Z . Clustering short time series gene expression data. Bioinformatics. 2005; 21 Suppl 1:i159-68. DOI: 10.1093/bioinformatics/bti1022. View

15.

Yang N, Zhang Q, Li J, Yang S, Shi Q . Progression of periodontal inflammation in adolescents is associated with increased number of Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, and Fusobacterium nucleatum. Int J Paediatr Dent. 2013; 24(3):226-33. DOI: 10.1111/ipd.12065. View

16.

Shoshan E, Braeuer R, Kamiya T, Mobley A, Huang L, Vasquez M . NFAT1 Directly Regulates IL8 and MMP3 to Promote Melanoma Tumor Growth and Metastasis. Cancer Res. 2016; 76(11):3145-55. PMC: 4891299. DOI: 10.1158/0008-5472.CAN-15-2511. View

17.

Shang F, Liu H . and colorectal cancer: A review. World J Gastrointest Oncol. 2018; 10(3):71-81. PMC: 5852398. DOI: 10.4251/wjgo.v10.i3.71. View

18.

Almstahl A, Wikstrom M . Oral microflora in subjects with reduced salivary secretion. J Dent Res. 1999; 78(8):1410-6. DOI: 10.1177/00220345990780080601. View

19.

Bardou P, Mariette J, Escudie F, Djemiel C, Klopp C . jvenn: an interactive Venn diagram viewer. BMC Bioinformatics. 2014; 15:293. PMC: 4261873. DOI: 10.1186/1471-2105-15-293. View

20.

Rossi A, Contiero J, da Silva Manoel-Caetano F, Severino F, Silva A . Up-regulation of tumor necrosis factor-α pathway survival genes and of the receptor TNFR2 in gastric cancer. World J Gastrointest Oncol. 2019; 11(4):281-294. PMC: 6475670. DOI: 10.4251/wjgo.v11.i4.281. View