Effect of Vitrification on Biogenesis Pathway and Expression of Development-related MicroRNAs in Preimplantation Mouse Embryos

Overview

Journal Cell Tissue Bank

Specialties Anatomy & Histology
Cell Biology
General Surgery

Date 2020 Oct 9

PMID 33033964

Citations 3

Authors

Elham Azizi

Marefat Ghaffari Novin

Mohammad Naji

Fardin Amidi

Hossein Hosseinirad

Zahra Shams Mofarahe

Affiliations

Soon will be listed here.

Abstract

Vitrification of embryos has been known as the most efficient cryopreservation method in assisted reproductive technology clinics. Vitrification of preimplantation embryo might be associated with altered gene expression profile and biochemical changes of vitrified embryos. Stringent regulation of gene expression in early embryonic stages is very critical for normal development. In the present study, we investigated the effect of vitrification on the canonical miRNA biogenesis pathway, and also the expression of developmental related miRNAs, in 8-cell and blastocyst mouse embryos. Although the expression pattern of the miRNA biogenesis pathway genes differed between 8-cell and blastocyst mouse embryos, vitrification did not affect the expression level of these genes in preimplantation embryos. The expression levels of miR-21 and let-7a were significantly decreased in vitrified 8-cell embryos and fresh blastocysts when compared with fresh 8-cell embryos. The expression of Stat3 was significantly reduced in blastocysts after vitrification. The alteration in the expression pattern of miRNAs, due to their mode of action, can affect broad downstream key developmental signaling pathways. Therefore, the blastocyst stage is the preferred point for embryo vitrification as they are less susceptible to cryo-damages regarding the stability of miRNAs related to the developmental and implantation competence of embryo.

Citing Articles

Transcriptomic Analysis of Vitrified-Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact.

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Evaluating the Differential Expression of miR-146a, miR-222, and miR-9 in Matched Serum and Follicular Fluid of Polycystic Ovary Syndrome Patients: Profiling and Predictive Value.

Ashrafnezhad Z, Naji M, Aleyasin A, Hedayatpour A, Mahdavinezhad F, Gharaei R Int J Mol Cell Med. 2023; 11(4):320-333.

PMID: 37727646 PMC: 10506678. DOI: 10.22088/IJMCM.BUMS.11.4.320.

Update on the Epigenomic Implication of Embryo Cryopreservation Methods Applied in Assisted Reproductive Technologies With Potential Long-Term Health Effects.

Palomares A, Rodriguez-Wallberg K Front Cell Dev Biol. 2022; 10:881550.

PMID: 35573677 PMC: 9096028. DOI: 10.3389/fcell.2022.881550.

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