Effect of Organic Compost and Inorganic Nitrogen Fertigation on Spinach Growth, Phytochemical Accumulation and Antioxidant Activity

Overview

Journal Heliyon

Specialty Social Sciences

Date 2020 Oct 7

PMID 33024866

Citations 9

Authors

R M A Machado

I Alves-Pereira

D Lourenco

R M A Ferreira

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to evaluate the effect of the addition of organic compost in combination with the inorganic nitrogen fertigation on growth, phytochemical accumulation, and antioxidant activity of spinach ( L. cv. Manatee). Soil blocked spinach seedlings (six seedlings per block), three blocks per pot (316 plants m) were transplanted after 18 days after emergence into to 12 L pots. The treatments were: unfertilized soil, organic compost, organic compost +75 kg of N ha, applied as ammonium sulfate; and organic compost +75 kg N ha, applied as ammonium nitrate. The addition of organic compost to unfertilized soil increased the fresh yield. The application of inorganic N from the two sources in relation to organic compost treatment increased spinach fresh yield from 2.3 to 4.81 kg m and shoot dry weight from 0.60 to 1,31 g plant. Levels of carotenoids also increased with inorganic N addition, producing higher values in plants grown with organic compost + ammonium nitrate (31.14 mg/100 g fresh weight). However, the addition of N led to a decrease in leaf-blade total phenols from 75 to 56 mg gallic acid equivalents/100mg fresh weight. The addition of inorganic N led to a dramatic decrease in leaf-blade ferric reducing antioxidant activity. This effect was higher with ammonium sulfate application. The application of organic compost and inorganic nitrogen had no influence on the petiole's phytochemical accumulation and antioxidant activity.

Citing Articles

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References

Favoino E, Hogg D . The potential role of compost in reducing greenhouse gases. Waste Manag Res. 2008; 26(1):61-9. DOI: 10.1177/0734242X08088584. View

Machado R, Alves-Pereira I, Ferreira R . Plant growth, phytochemical accumulation and antioxidant activity of substrate-grown spinach. Heliyon. 2018; 4(8):e00751. PMC: 6106698. DOI: 10.1016/j.heliyon.2018.e00751. View

Sreeramulu D, Reddy C, Chauhan A, Balakrishna N, Raghunath M . Natural antioxidant activity of commonly consumed plant foods in India: effect of domestic processing. Oxid Med Cell Longev. 2013; 2013:369479. PMC: 3694554. DOI: 10.1155/2013/369479. View

Bouayed J, Hoffmann L, Bohn T . Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: Bioaccessibility and potential uptake. Food Chem. 2014; 128(1):14-21. DOI: 10.1016/j.foodchem.2011.02.052. View

Marmo L . EU strategies and policies on soil and waste management to offset greenhouse gas emissions. Waste Manag. 2007; 28(4):685-9. DOI: 10.1016/j.wasman.2007.09.030. View

Gutierrez-Rodriguez E, Lieth H, Jernstedt J, Labavitch J, Suslow T, Cantwell M . Texture, composition and anatomy of spinach leaves in relation to nitrogen fertilization. J Sci Food Agric. 2012; 93(2):227-37. DOI: 10.1002/jsfa.5780. View

Nguyen P, Niemeyer E . Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of basil (Ocimum basilicum L.). J Agric Food Chem. 2008; 56(18):8685-91. DOI: 10.1021/jf801485u. View

Qadir O, Siervo M, Seal C, Brandt K . Manipulation of Contents of Nitrate, Phenolic Acids, Chlorophylls, and Carotenoids in Lettuce (Lactuca sativa L.) via Contrasting Responses to Nitrogen Fertilizer When Grown in a Controlled Environment. J Agric Food Chem. 2017; 65(46):10003-10010. DOI: 10.1021/acs.jafc.7b03675. View

Liu X, Wu F, Li J, Chen J, Wang G, Wang W . Glutathione homeostasis and Cd tolerance in the Arabidopsis sultr1;1-sultr1;2 double mutant with limiting sulfate supply. Plant Cell Rep. 2015; 35(2):397-413. DOI: 10.1007/s00299-015-1892-8. View

10.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

11.

Hassan S, Mijin S, Yusoff U, Ding P, Megat Wahab P . Nitrate, ascorbic acid, mineral and antioxidant activities of Cosmos caudatus in response to organic and mineral-based fertilizer rates. Molecules. 2012; 17(7):7843-53. PMC: 6268055. DOI: 10.3390/molecules17077843. View

12.

Dominguez-Valdivia M, Aparicio-Tejo P, Lamsfus C, Cruz C, Martins-Loucao M, Moran J . Nitrogen nutrition and antioxidant metabolism in ammonium-tolerant and -sensitive plants. Physiol Plant. 2008; 132(3):359-69. DOI: 10.1111/j.1399-3054.2007.01022.x. View

13.

Dewhirst R, Clarkson G, Rothwell S, Fry S . Novel insights into ascorbate retention and degradation during the washing and post-harvest storage of spinach and other salad leaves. Food Chem. 2017; 233:237-246. PMC: 5441274. DOI: 10.1016/j.foodchem.2017.04.082. View

14.

Scheible W, Morcuende R, Czechowski T, Fritz C, Osuna D, Palacios-Rojas N . Genome-wide reprogramming of primary and secondary metabolism, protein synthesis, cellular growth processes, and the regulatory infrastructure of Arabidopsis in response to nitrogen. Plant Physiol. 2004; 136(1):2483-99. PMC: 523316. DOI: 10.1104/pp.104.047019. View

15.

Boldrin A, Andersen J, Moller J, Christensen T, Favoino E . Composting and compost utilization: accounting of greenhouse gases and global warming contributions. Waste Manag Res. 2009; 27(8):800-12. DOI: 10.1177/0734242X09345275. View

16.

Guo X, Liu H, Wu S . Humic substances developed during organic waste composting: Formation mechanisms, structural properties, and agronomic functions. Sci Total Environ. 2019; 662:501-510. DOI: 10.1016/j.scitotenv.2019.01.137. View

17.

Ibrahim M, Jaafar H, Rahmat A, Rahman Z . The relationship between phenolics and flavonoids production with total non structural carbohydrate and photosynthetic rate in Labisia pumila Benth. under high CO2 and nitrogen fertilization. Molecules. 2010; 16(1):162-74. PMC: 6259453. DOI: 10.3390/molecules16010162. View

18.

Luwe M, Takahama U, Heber U . Role of Ascorbate in Detoxifying Ozone in the Apoplast of Spinach (Spinacia oleracea L.) Leaves. Plant Physiol. 1993; 101(3):969-976. PMC: 158714. DOI: 10.1104/pp.101.3.969. View

19.

Wu W, Ma B . Integrated nutrient management (INM) for sustaining crop productivity and reducing environmental impact: a review. Sci Total Environ. 2015; 512-513:415-427. DOI: 10.1016/j.scitotenv.2014.12.101. View

20.

Hissin P, HILF R . A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem. 1976; 74(1):214-26. DOI: 10.1016/0003-2697(76)90326-2. View