Snake C-type Lectin-like Proteins Inhibit Nicotinic Acetylcholine Receptors

Overview

Journal J Venom Res

Date 2020 Oct 7

PMID 33024544

Citations 1

Authors

Elena V Kryukova

Catherine A Vulfius

Rustam H Ziganshin

Tatyana V Andreeva

Vladislav G Starkov

Victor I Tsetlin

Yuri N Utkin

Affiliations

Soon will be listed here.

Abstract

Venoms of viperid snakes affect mostly hemostasis, while C-type lectin-like proteins (CTLPs), one of the main components of viperid venoms, act as anticoagulants, procoagulants, or agonists/antagonists of platelet activation. However, we have shown earlier that CTLPs from the saw-scaled viper , called emunarecins EM1 and EM2, were able to inhibit nicotinic acetylcholine receptors (nAChRs) in neurons of a pond snail (). Here we analysed the structure of the emunarecins by mass spectrometry and report that EM1 and EM2 inhibit fluorescent α-bungarotoxin binding to both muscle-type nAChRs from and human neuronal α7 nAChRs. EM1 at 23µM and EM2 at 9µM almost completely prevented fluorecsent α-bungarotoxin binding to muscle-type nAChRs. Interaction with human neuronal α7 nAChR was weaker; EM1 at the concentration of 23µM blocked the α-bungarotoxin binding only by about 40% and EM2 at 9µM by about 20%. The efficiency of the EM2 interaction with nAChRs was comparable to that of a non-conventional toxin, WTX, from cobra venom. Together with the data obtained earlier, these results show that CTLPs may represent new nAChR ligands.

Citing Articles

What Are the Neurotoxins in Hemotoxic Snake Venoms?.

Osipov A, Utkin Y Int J Mol Sci. 2023; 24(3).

PMID: 36769242 PMC: 9917609. DOI: 10.3390/ijms24032919.

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