Effect of Bariatric Surgery on Circulating and Urinary Mitochondrial DNA Copy Numbers in Obesity with or Without Diabetes

Overview

Journal BMJ Open Diabetes Res Care

Specialty Endocrinology

Date 2020 Oct 6

PMID 33020132

Citations 6

Authors

Mihae Seo

Hyoungnae Kim

Hyunjin Noh

Jin Seok Jeon

Dong Won Byun

Sang Hyun Kim

Hye Jeong Kim

Kyoil Suh

Hyeong Kyu Park

Soon Hyo Kwon

Affiliations

Soon will be listed here.

Abstract

Introduction: Recent studies have suggested that extracellular circulating and urinary mitochondrial DNA (mtDNA) are associated with mitochondrial dysfunction in obesity and type 2 diabetes mellitus (T2DM). However, the changes to cell-free serum and urinary mtDNA after bariatric surgery in patients with obesity with T2DM have not been investigated to date.

Research Design And Methods: We prospectively recruited patients with obesity (n=18), and with obesity and T2DM (n=14) who underwent bariatric surgery, along with healthy volunteers (HV) as a control group (n=22). Serum and urinary mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunit-1 (mtND-1) and cytochrome-c oxidase 3 (mtCOX-3) copy numbers were measured using quantitative PCR (qPCR). The mtDNA copy numbers of patients with obesity (with and without T2DM) were followed up 6 months after surgery.

Results: The copy numbers of urinary mtND-1 and mtCOX-3 in patients with obesity, with or without T2DM, were higher than those in the HVs. Moreover, urinary mtCOX-3 copy number increased in patients with obesity with T2DM compared with patients with obesity without T2DM (p=0.018). Meanwhile, serum mtCOX-3 copy numbers in HV were higher in both obesity patient groups (p=0.040). Bariatric surgery reduced urinary mtND-1 and mtCOX-3 copy numbers, as well as serum mtCOX-3 copy numbers only in patients with obesity with T2DM.

Conclusion: These results suggest that T2DM induces greater kidney mitochondrial dysfunction in patients with obesity, which can be effectively restored with bariatric surgery.

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