Extended Spectrum Beta-lactamase and Fluoroquinolone Resistance Genes Among Escherichia Coli and Salmonella Isolates from Children with Diarrhea, Burkina Faso

Overview

Journal BMC Pediatr

Publisher Biomed Central

Specialty Pediatrics

Date 2020 Oct 4

PMID 33010801

Citations 8

Authors

Rene Dembele

Ali Konate

Oumar Traore

Wendpoulomde A D Kabore

Issiaka Soulama

Asseta Kagambega

Alfred S Traore

Nathalie K Guessennd

Awa Aidara-Kane

Amy Gassama-Sow

Nicolas Barro

Affiliations

Soon will be listed here.

Abstract

Background: The emergence and spread of multidrug-resistant gram-negative bacteria (MDR) has become a major public health concern worldwide. This resistance is caused by enzymes-mediated genes (i.e., extended spectrum beta-lactamases) that are common in certain Enterobacterioceae species. However, the distribution of these genes is poorly documented in Burkina Faso. This study aims to determine the prevalence and distribution of the resistant genes coding for broad spectrum beta-lactamases and quinolones in rural Burkina Faso.

Methods: Multiplex PCR assays were carried out to detect ESBL-encoding genes, including bla, bla, bla, bla. The assays also assessed the presence of quinolone resistance gene namely qnrA, qnrB and qnrS in the quinolone-resistance DEC and Salmonella strains.

Results: The Extended-Spectrum Beta-Lactamases (ESBL) resistance phenotype was reported in all the E. coli isolates (5/5). Cross-resistance phenotype to quinolones (CRQ) was shown by one Salmonella strain (1/9) and three E. coli (3/5). Cross-resistance phenotypes to fluoroquinolones (CRFQ) were harboured by one Salmonella (1/9) and carbapenemase phenotypes were detected in two E. coli strains (2/5). Whilst the bla genes were detected in 100% (5/5) of E. coli isolates and in 33.33% (3/9) Salmonella isolates. One strain of E. coli (1/5) harbored the bla gene and the qnrB gene simultaneously.

Conclusions: This study identified β-lactam (bla) and quinolone resistance (qnr) genes in multidrug-resistant E. coli and Salmonella spp. in rural Burkina Faso. Our finding which highlighted the enterobacteriaceae strains resistance to β-lactams and quinolones are of high interest for adequate management of antimicrobial resistant genes outbreak in Burkina Faso.

Citing Articles

Occurrence of Extended-spectrum β-lactamase (ESBL) and Carbapenemase-producing Escherichia coli isolated from Childhood Diarrhoea in Yaoundé, Cameroon.

Mahamat S, Founou R, Founou L, Tchouangueu T, Dimani B, Nkengkana O BMC Microbiol. 2024; 24(1):401.

PMID: 39385062 PMC: 11465673. DOI: 10.1186/s12866-024-03559-z.

Isolation and Characterization of Environmental Extended Spectrum β-Lactamase-Producing and from Ouagadougou, Burkina Faso.

Kagambega A, Dembele R, Traore O, Wane A, Mohamed A, Coulibaly H Pharmaceuticals (Basel). 2024; 17(3).

PMID: 38543091 PMC: 10974332. DOI: 10.3390/ph17030305.

Epidemiology of Cefotaxime-Hydrolysing β-Lactamase-Producing in Children with Diarrhoea Reported Globally between 2012 and 2022.

Karambwe S, Traore A, Potgieter N Microorganisms. 2024; 12(1).

PMID: 38257997 PMC: 10820611. DOI: 10.3390/microorganisms12010171.

Contribution of the Rapid LAMP-Based Diagnostic Test (RLDT) to the Evaluation of Enterotoxigenic (ETEC) and in Childhood Diarrhea in the Peri-Urban Area of Ouagadougou, Burkina Faso.

Hema A, Serme S, Sawadogo J, Diarra A, Barry A, Ouedraogo A Microorganisms. 2023; 11(11).

PMID: 38004821 PMC: 10673293. DOI: 10.3390/microorganisms11112809.

Epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli at the human-animal-environment interface in a farming community of central Uganda.

Muleme J, Musoke D, Balugaba B, Kisaka S, Makumbi F, Buregyeya E PLOS Glob Public Health. 2023; 3(6):e0001344.

PMID: 37310955 PMC: 10270331. DOI: 10.1371/journal.pgph.0001344.

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