Incrimination of and As Vectors of Dengue Virus Serotypes 1, 2 and 3 from Four States of Northeast India

Overview

Journal Access Microbiol

Specialty Microbiology

Date 2020 Oct 2

PMID 33005866

Citations 5

Authors

Sumi Chetry

Saurav Jyoti Patgiri

Dibya Ranjan Bhattacharyya

Prafulla Dutta

N Pradeep Kumar

Affiliations

Soon will be listed here.

Abstract

Dengue is an important vector borne disease with a great public health concern worldwide. Northeast India has experienced dengue almost every year for a decade. As studies on dengue vectors from this region are limited, we undertook an investigation to detect natural infection of the dengue virus (DENV) in potential dengue vectors of this region. Adult mosquitoes which were collected were subjected to RT-PCR for detection of infecting dengue serotype. Minimum infection rate was also determined for each positive pool. Out of the total 6229 adult mosquitoes collected, (63.3 %) was abundant in comparison to (36.7 %). These specimens (515 mosquito pools) were subjected to RT-PCR for detection of DENV-1, 2, 3 and 4. RT-PCR revealed the existence of DENV in both male as well as female mosquito pools suggesting natural transovarial transmission of DENV in this region. A total of 54 pools tested were positive for DENV-1, 2, 3 serotypes. This study revealed the occurence of DENV in both the potential dengue vectors from this region along with evidence of transovarial transmission which helps in persistence of the virus in nature.

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