Screening of Subsp. with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Oct 2

PMID 33003556

Citations 24

Authors

Weiwei Wang

Hao Ma

Haojie Yu

Guangyong Qin

Zhongfang Tan

Yanping Wang

Huili Pang

Affiliations

Soon will be listed here.

Abstract

For screening excellent lactic acid bacteria (LAB) strains to inhibit enterotoxigenic (ETEC) K88, inhibitory activities of more than 1100 LAB strains isolated from different materials, and kept in the lab, were evaluated in this study. Nine strains with inhibition zones, at least 22.00 mm (including that of a hole puncher, 10.00 mm), and good physiological and biochemical characteristics identified by 16S DNA gene sequencing and A gene multiple detection, were assigned to () subsp. (5), (1), (1), (1) and (1), respectively. As investigated for their tolerance abilities and safety, only strain ZA3 possessed high hydrophobicity and auto-aggregation abilities, had high survival rate in low pH, bile salt environment, and gastrointestinal (GI) fluids, was sensitive to ampicillin, and resistant to norfloxacin and amikacin, without hemolytic activity, and did not carry antibiotic resistance genes, but exhibited broad spectrum activity against a wide range of microorganisms. Antibacterial substance may attribute to organic acids, especially lactic acid and acetic acid. The results indicated that the selected strain subsp. ZA3 could be considered a potential probiotic to inhibit ETEC K88 in weaned piglets for further research.

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References

Mingmongkolchai S, Panbangred W . Bacillus probiotics: an alternative to antibiotics for livestock production. J Appl Microbiol. 2018; 124(6):1334-1346. DOI: 10.1111/jam.13690. View

Fuller R . Probiotics in man and animals. J Appl Bacteriol. 1989; 66(5):365-78. View

Joghataei M, Shahidi F, Pouladfar G, Mortazavi S, Ghaderi A . Probiotic potential comparison of Lactobacillus strains isolated from Iranian traditional food products and human feces with standard probiotic strains. J Sci Food Agric. 2019; 99(15):6680-6688. DOI: 10.1002/jsfa.9945. View

Somashekaraiah R, Shruthi B, Deepthi B, Sreenivasa M . Probiotic Properties of Lactic Acid Bacteria Isolated From Neera: A Naturally Fermenting Coconut Palm Nectar. Front Microbiol. 2019; 10:1382. PMC: 6611078. DOI: 10.3389/fmicb.2019.01382. View

Atassi F, Servin A . Individual and co-operative roles of lactic acid and hydrogen peroxide in the killing activity of enteric strain Lactobacillus johnsonii NCC933 and vaginal strain Lactobacillus gasseri KS120.1 against enteric, uropathogenic and vaginosis-associated.... FEMS Microbiol Lett. 2010; 304(1):29-38. DOI: 10.1111/j.1574-6968.2009.01887.x. View

Tirloni E, Bernardi C, Ghelardi E, Celandroni F, Andrighetto C, Rota N . Biopreservation as a potential hurdle for Bacillus cereus growth in fresh cheese. J Dairy Sci. 2019; 103(1):150-160. DOI: 10.3168/jds.2019-16739. View

Zhang L, Xu Y, Liu H, Lai T, Ma J, Wang J . Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea: Effects on diarrhoea incidence, faecal microflora and immune responses. Vet Microbiol. 2009; 141(1-2):142-8. DOI: 10.1016/j.vetmic.2009.09.003. View

Wang S, Dong Z, Li J, Chen L, Shao T . Pediococcus acidilactici strains as silage inoculants for improving the fermentation quality, nutritive value and in vitro ruminal digestibility in different forages. J Appl Microbiol. 2018; 126(2):424-434. DOI: 10.1111/jam.14146. View

Rao Y, Tao Y, Li Y, She X, Yang J, Qian Y . Characterization of a probiotic starter culture with anti-Candida activity for Chinese pickle fermentation. Food Funct. 2019; 10(10):6936-6944. DOI: 10.1039/c9fo01191a. View

10.

Sterndale S, Evans D, Mansfield J, Clarke J, Sahibzada S, Abraham S . Effect of mucin 4 allele on susceptibility to experimental infection with enterotoxigenic F4 in pigs fed experimental diets. J Anim Sci Biotechnol. 2019; 10:56. PMC: 6636048. DOI: 10.1186/s40104-019-0366-1. View

11.

Sirichokchatchawan W, Pupa P, Praechansri P, Am-In N, Tanasupawat S, Sonthayanon P . Autochthonous lactic acid bacteria isolated from pig faeces in Thailand show probiotic properties and antibacterial activity against enteric pathogenic bacteria. Microb Pathog. 2018; 119:208-215. DOI: 10.1016/j.micpath.2018.04.031. View

12.

Nawaz M, Wang J, Zhou A, Ma C, Wu X, Moore J . Characterization and transfer of antibiotic resistance in lactic acid bacteria from fermented food products. Curr Microbiol. 2011; 62(3):1081-9. DOI: 10.1007/s00284-010-9856-2. View

13.

Kumara S, Bashisht A, Venkateswaran G, Hariprasad P, Gayathri D . Characterization of Novel Lactobacillus fermentum from Curd Samples of Indigenous Cows from Malnad Region, Karnataka, for their Aflatoxin B Binding and Probiotic Properties. Probiotics Antimicrob Proteins. 2018; 11(4):1100-1109. DOI: 10.1007/s12602-018-9479-7. View

14.

Boyle R, Robins-Browne R, Tang M . Probiotic use in clinical practice: what are the risks?. Am J Clin Nutr. 2006; 83(6):1256-64. DOI: 10.1093/ajcn/83.6.1256. View

15.

Fleckenstein J, Kuhlmann F . Enterotoxigenic Escherichia coli Infections. Curr Infect Dis Rep. 2019; 21(3):9. PMC: 6508951. DOI: 10.1007/s11908-019-0665-x. View

16.

Konstantinov S, Smidt H, Akkermans A, Casini L, Trevisi P, Mazzoni M . Feeding of Lactobacillus sobrius reduces Escherichia coli F4 levels in the gut and promotes growth of infected piglets. FEMS Microbiol Ecol. 2008; 66(3):599-607. DOI: 10.1111/j.1574-6941.2008.00517.x. View

17.

Dlamini Z, Langa R, Aiyegoro O, Okoh A . Safety Evaluation and Colonisation Abilities of Four Lactic Acid Bacteria as Future Probiotics. Probiotics Antimicrob Proteins. 2018; 11(2):397-402. DOI: 10.1007/s12602-018-9430-y. View

18.

Rhouma M, Fairbrother J, Beaudry F, Letellier A . Post weaning diarrhea in pigs: risk factors and non-colistin-based control strategies. Acta Vet Scand. 2017; 59(1):31. PMC: 5437690. DOI: 10.1186/s13028-017-0299-7. View

19.

Dushku E, Ioannou A, Staikou A, Yiangou M . Probiotic properties and immunomodulatory activity of gastrointestinal tract commensal bacterial strains isolated from the edible farmed snail Cornu aspersum maxima. Fish Shellfish Immunol. 2019; 92:792-801. DOI: 10.1016/j.fsi.2019.06.061. View

20.

Bah A, Albano H, Barbosa J, Fhoula I, Gharbi Y, Najjari A . Inhibitory Effect of FL75 and FL14 Foodborne Pathogens in Artificially Contaminated Fermented Tomato Juices. Biomed Res Int. 2019; 2019:6937837. PMC: 6413396. DOI: 10.1155/2019/6937837. View