Comparison of Enzyme-linked Immunosorbent Assay and Complement Fixation and Indirect Fluorescent-antibody Tests for Detection of Coxiella Burnetii Antibody

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1987 Jun 1

PMID 3298311

Citations 25

Authors

O Peter

G Dupuis

M G Peacock

W BURGDORFER

Affiliations

Soon will be listed here.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G to Coxiella burnetii phase II. Serum samples from 213 patients who had had Q fever 1 year previously and from 301 blood donors from six localities in Switzerland were tested by ELISA and by indirect fluorescent-antibody (IFA) and complement fixation (CF) tests. The ELISA and the IFA and CF tests detected antibody to C. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 Q fever patients, respectively. With the serum samples from blood donors, the ELISA yielded a higher percentage of positive sera than did the IFA and CF tests. The high specificity of the three tests was confirmed by analyzing paired serum samples from 36 patients suffering from acute pneumonia of viral or bacterial origin. In these cases, the serological results were negative by the three tests, except for three Q-fever cases included as positive control.

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References

Turck W, Howitt G, Turnberg L, Fox H, Longson M, MATTHEWS M . Chronic Q fever. Q J Med. 1976; 45(178):193-217. View

Field P, Hunt J, Murphy A . Detection and persistence of specific IgM antibody to Coxiella burnetii by enzyme-linked immunosorbent assay: a comparison with immunofluorescence and complement fixation tests. J Infect Dis. 1983; 148(3):477-87. DOI: 10.1093/infdis/148.3.477. View

Doller G, Doller P, GERTH H . Early diagnosis of Q fever: detection of immunoglobulin M by radioimmunoassay and enzyme immunoassay. Eur J Clin Microbiol. 1984; 3(6):550-3. DOI: 10.1007/BF02013617. View

WIESMANN E . [Q fever research in Switzerland in the years 1947-1951]. Z Tropenmed Parasitol. 1952; 3(3):297-301. View

COX H . CULTIVATION OF RICKETTSIAE OF THE ROCKY MOUNTAIN SPOTTED FEVER, TYPHUS AND Q FEVER GROUPS IN THE EMBRYONIC TISSUES OF DEVELOPING CHICKS. Science. 1941; 94(2444):399-403. DOI: 10.1126/science.94.2444.399. View

Clements M, Dumler J, Fiset P, WISSEMAN Jr C, Snyder M, Levine M . Serodiagnosis of Rocky Mountain spotted fever: comparison of IgM and IgG enzyme-linked immunosorbent assays and indirect fluorescent antibody test. J Infect Dis. 1983; 148(5):876-80. DOI: 10.1093/infdis/148.5.876. View

Roges G, EDLINGER E . Immunoenzymatic test for Q-fever. Diagn Microbiol Infect Dis. 1986; 4(2):125-32. DOI: 10.1016/0732-8893(86)90146-x. View

Peacock M, PHILIP R, Williams J, FAULKNER R . Serological evaluation of O fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis. Infect Immun. 1983; 41(3):1089-98. PMC: 264612. DOI: 10.1128/iai.41.3.1089-1098.1983. View

Dasch G, Halle S, Bourgeois A . Sensitive microplate enzyme-linked immunosorbent assay for detection of antibodies against the scrub typhus rickettsia, Rickettsia tsutsugamushi. J Clin Microbiol. 1979; 9(1):38-48. PMC: 272954. DOI: 10.1128/jcm.9.1.38-48.1979. View

10.

Halle S, Dasch G, Weiss E . Sensitive enzyme-linked immunosorbent assay for detection of antibodies against typhus rickettsiae, Rickettsia prowazekii and Rickettsia typhi. J Clin Microbiol. 1977; 6(2):101-10. PMC: 274714. DOI: 10.1128/jcm.6.2.101-110.1977. View

11.

Dupuis G, Peter O, Mottiez M, Vouilloz M . [Seroprevalence of human Q fever in Switzerland]. Schweiz Med Wochenschr. 1986; 116(16):494-8. View

12.

WIESMANN E, Schweizer R, Tobler H . [Q fever in North-East Switzerland; study of the winter 1954-55 epidemic]. Schweiz Med Wochenschr. 1956; 86(3):60-3. View

13.

Haldane E, Marrie T, FAULKNER R, Lee S, COOPER J, Macpherson D . Endocarditis due to Q fever in Nova Scotia: experience with five patients in 1981-1982. J Infect Dis. 1983; 148(6):978-85. DOI: 10.1093/infdis/148.6.978. View

14.

Baca O, PARETSKY D . Q fever and Coxiella burnetii: a model for host-parasite interactions. Microbiol Rev. 1983; 47(2):127-49. PMC: 281568. DOI: 10.1128/mr.47.2.127-149.1983. View

15.

Peter O, Dupuis G, BURGDORFER W, Peacock M . Evaluation of the complement fixation and indirect immunofluorescence tests in the early diagnosis of primary Q fever. Eur J Clin Microbiol. 1985; 4(4):394-6. DOI: 10.1007/BF02148690. View

16.

Dupuis G, Peter O, Pedroni D, Petite J . [Clinical aspects observed during an epidemic of 415 cases of Q fever]. Schweiz Med Wochenschr. 1985; 115(24):814-8. View

17.

Amano K, Williams J . Chemical and immunological characterization of lipopolysaccharides from phase I and phase II Coxiella burnetii. J Bacteriol. 1984; 160(3):994-1002. PMC: 215808. DOI: 10.1128/jb.160.3.994-1002.1984. View

18.

PHILIP R, CASPER E, ORMSBEE R, Peacock M, BURGDORFER W . Microimmunofluorescence test for the serological study of rocky mountain spotted fever and typhus. J Clin Microbiol. 1976; 3(1):51-61. PMC: 274226. DOI: 10.1128/jcm.3.1.51-61.1976. View