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Two Distinct Transpeptidation Reactions During Murein Synthesis in Escherichia Coli

Overview
Journal J Bacteriol
Specialty Microbiology
Date 1987 Jul 1
PMID 3298212
Citations 19
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Abstract

Murein synthesized in ether-permeabilized cells of Escherichia coli deficient in individual penicillin-binding proteins (PBPs) and in the presence of certain beta-lactam antibiotics was analyzed by high-pressure liquid chromatography separation of the muramidase split products. PBP 1b was found to to be the major murein synthesizing activity that was poorly compensated for by PBP 1a. A PBP 2 mutant as well as mecillinam-inhibited cells showed increased activity in the formation of oligomeric muropeptides as well as UDP-muramylpeptidyl-linked muropeptides, the reaction products of transpeptidation, bypassing the lipid intermediate. In contrast, penicillin G and furazlocillin severely inhibited these reactions but stimulated normal dimer production. It is concluded that two distinct transpeptidases exist in E. coli: one, highly sensitive to penicillin G and furazlocillin, catalyzes the formation of hyper-cross-linked muropeptides, and a second one, quite resistant to these antibiotics, synthesizes muropeptide dimers.

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References
1.
Spratt B . Deletion of the penicillin-binding protein 5 gene of Escherichia coli. J Bacteriol. 1980; 144(3):1190-2. PMC: 294790. DOI: 10.1128/jb.144.3.1190-1192.1980. View

2.
Suzuki H, Van Heijenoort Y, Tamura T, Mizoguchi J, Hirota Y, van Heijenoort J . In vitro peptidoglycan polymerization catalysed by penicillin binding protein 1b of Escherichia coli K-12. FEBS Lett. 1980; 110(2):245-9. DOI: 10.1016/0014-5793(80)80083-4. View

3.
Ishino F, Matsuhashi M . Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence. Biochem Biophys Res Commun. 1981; 101(3):905-11. DOI: 10.1016/0006-291x(81)91835-0. View

4.
Maass D, Pelzer H . Murein biosynthesis in ether permeabilized Escherichia coli starting from early peptidoglycan precursors. Arch Microbiol. 1981; 130(4):301-6. DOI: 10.1007/BF00425944. View

5.
Nakagawa J, Matsuhashi M . Molecular divergence of a major peptidoglycan synthetase with transglycosylase-transpeptidase activities in Escherichia coli --- penicillin-binding protein 1Bs. Biochem Biophys Res Commun. 1982; 105(4):1546-53. DOI: 10.1016/0006-291x(82)90964-0. View