SERRS Multiplexing with Multivalent Nanostructures for the Identification and Enumeration of Epithelial and Mesenchymal Cells

Overview

Journal Sci Rep

Specialty Science

Date 2020 Sep 26

PMID 32978492

Citations 8

Authors

Lucio Litti

Andrea Colusso

Marcella Pinto

Erlis Ruli

Alessia Scarsi

Laura Ventura

Giuseppe Toffoli

Marco Colombatti

Giulio Fracasso

Moreno Meneghetti

Affiliations

Soon will be listed here.

Abstract

Liquid biopsy represents a new frontier of cancer diagnosis and prognosis, which allows the isolation of tumor cells released in the blood stream. The extremely low abundance of these cells needs appropriate methodologies for their identification and enumeration. Herein we present a new protocol based on surface enhanced resonance Raman scattering (SERRS) gold multivalent nanostructures to identify and enumerate tumor cells with epithelial and mesenchimal markers. The validation of the protocol is obtained with spiked samples of peripheral blood mononuclear cells (PBMC). Gold nanostructures are functionalized with SERRS labels and with antibodies to link the tumor cells. Three types of such nanosystems were simultaneously used and the protocol allows obtaining the identification of all individual tumor cells with the help of a Random Forest ensemble learning method.

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