Quantitative Proteomic Analysis of the Expression of SARS-CoV-2 Receptors in the Gut of Patients with Chronic Enterocolitis

Overview

Journal Yonsei Med J

Specialty General Medicine

Date 2020 Sep 25

PMID 32975064

Citations 4

Authors

Jihye Park

Daeun Jeong

Youn Wook Chung

Da Hye Kim

Jae Hee Cheon

Ji Hwan Ryu

Affiliations

Soon will be listed here.

Abstract

The cellular entry of severe respiratory syndrome coronavirus-2 (SARS-CoV-2) is mediated by interaction with the human angiotensin-converting enzyme 2 (ACE2), a receptor that is expressed on both lung and intestinal epithelial cells. We performed a quantitative proteomic analysis to investigate the expression of possible receptors for SARS-CoV-2 in the intestinal mucosa of 23 patients with chronic colitis. ACE2 expression was low and remained unaltered in the gut of patients with ulcerative colitis (UC), Crohn's disease (CD), intestinal Behćet's disease (BD), and intestinal tuberculosis (TB), when compared with that of healthy individuals. Additionally, the expression levels of some probable co-receptors, including dipeptidyl peptidase 4 (DPP4), aminopeptidase N (AMPN), and glutamyl aminopeptidase (AMPE), were unchanged in the affected UC, CD, intestinal BD, and intestinal TB colon mucosa samples. In conclusion, gut inflammation associated with chronic colitis does not mediate a further increase in the cellular entry of SARS-CoV-2.

Citing Articles

Altered plasma levels of the SARS-CoV-2-related proteins ACE2 and TMPRSS2 in patients with Crohn's disease.

Saez-Leyva J, Lennol M, Aviles-Granados C, Garcia-Ayllon M, Gutierrez A, Frances R Sci Rep. 2024; 14(1):30346.

PMID: 39638806 PMC: 11621418. DOI: 10.1038/s41598-024-81810-3.

The enzymes in COVID-19: A review.

Alves M, Mahnke L, Macedo T, Silva T, Carvalho Junior L Biochimie. 2022; 197:38-48.

PMID: 35090981 PMC: 8789385. DOI: 10.1016/j.biochi.2022.01.015.

Korean Association for the Study of Intestinal Diseases guidance for clinical practice of adult inflammatory bowel disease during the coronavirus disease 2019 pandemic: expert consensus statements.

Park Y, Lee Y, Chang J, Song H, Kim D, Yang Y Intest Res. 2022; 20(4):431-444.

PMID: 34974675 PMC: 9650326. DOI: 10.5217/ir.2021.00111.

Could protein content of Urinary Extracellular Vesicles be useful to detect Cirrhosis in Alcoholic Liver Disease?.

Gonzalez E, Azkargorta M, Garcia-Vallicrosa C, Prieto-Elordui J, Elortza F, Blanco-Sampascual S Int J Biol Sci. 2021; 17(8):1864-1877.

PMID: 34131392 PMC: 8193259. DOI: 10.7150/ijbs.59725.

References

An P, Ji M, Ren H, Su J, Ding N, Kang J . Prevention of COVID-19 in patients with inflammatory bowel disease in Wuhan, China. Lancet Gastroenterol Hepatol. 2020; 5(6):525-527. PMC: 7164865. DOI: 10.1016/S2468-1253(20)30121-7. View

Harmer D, Gilbert M, Borman R, Clark K . Quantitative mRNA expression profiling of ACE 2, a novel homologue of angiotensin converting enzyme. FEBS Lett. 2002; 532(1-2):107-10. DOI: 10.1016/s0014-5793(02)03640-2. View

Guan W, Ni Z, Hu Y, Liang W, Ou C, He J . Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med. 2020; 382(18):1708-1720. PMC: 7092819. DOI: 10.1056/NEJMoa2002032. View

Imai Y, Kuba K, Rao S, Huan Y, Guo F, Guan B . Angiotensin-converting enzyme 2 protects from severe acute lung failure. Nature. 2005; 436(7047):112-6. PMC: 7094998. DOI: 10.1038/nature03712. View

Qi F, Qian S, Zhang S, Zhang Z . Single cell RNA sequencing of 13 human tissues identify cell types and receptors of human coronaviruses. Biochem Biophys Res Commun. 2020; 526(1):135-140. PMC: 7156119. DOI: 10.1016/j.bbrc.2020.03.044. View

Ning L, Shan G, Sun Z, Zhang F, Xu C, Lou X . Quantitative Proteomic Analysis Reveals the Deregulation of Nicotinamide Adenine Dinucleotide Metabolism and CD38 in Inflammatory Bowel Disease. Biomed Res Int. 2019; 2019:3950628. PMC: 6507272. DOI: 10.1155/2019/3950628. View

Hoffmann M, Kleine-Weber H, Schroeder S, Kruger N, Herrler T, Erichsen S . SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020; 181(2):271-280.e8. PMC: 7102627. DOI: 10.1016/j.cell.2020.02.052. View

Xiao F, Tang M, Zheng X, Liu Y, Li X, Shan H . Evidence for Gastrointestinal Infection of SARS-CoV-2. Gastroenterology. 2020; 158(6):1831-1833.e3. PMC: 7130181. DOI: 10.1053/j.gastro.2020.02.055. View

Patel A, Verma A . Nasal ACE2 Levels and COVID-19 in Children. JAMA. 2020; 323(23):2386-2387. DOI: 10.1001/jama.2020.8946. View

10.

Lee C, Lee K, Park D, Jung S, Jeen Y, Park Y . A new opportunity for innovative inflammatory bowel disease research: the moderate-to-severe ulcerative colitis in Korea (MOSAIK) cohort study. Intest Res. 2019; 17(1):1-5. PMC: 6361028. DOI: 10.5217/ir.2019.00005. View

11.

Garg M, Royce S, Tikellis C, Shallue C, Batu D, Velkoska E . Imbalance of the renin-angiotensin system may contribute to inflammation and fibrosis in IBD: a novel therapeutic target?. Gut. 2019; 69(5):841-851. DOI: 10.1136/gutjnl-2019-318512. View

12.

Goyal P, Choi J, Pinheiro L, Schenck E, Chen R, Jabri A . Clinical Characteristics of Covid-19 in New York City. N Engl J Med. 2020; 382(24):2372-2374. PMC: 7182018. DOI: 10.1056/NEJMc2010419. View