Resistance of a Halobacterium Salinarum Isolate from a Solar Saltern to Cadmium, Lead, Nickel, Zinc, and Copper

Overview

Journal Antonie Van Leeuwenhoek

Publisher Springer

Specialty Microbiology

Date 2020 Sep 25

PMID 32974806

Citations 7

Authors

Houda Baati

Mariem Siala

Chafai Azri

Emna Ammar

Christopher Dunlap

Mohamed Trigui

Affiliations

Soon will be listed here.

Abstract

The current study focuses on the tolerance of a strain of Halobacterium salinarum isolated from Sfax solar saltern (Tunisia) towards cadmium (Cd), lead (Pb), nickel (Ni), zinc (Zn), and copper (Cu) by using agar dilution methods in complex and minimal media. The results showed the least inhibitory metals based on Minimum Inhibitory Concentrations (MICs) were lead (MIC = 4.5 mM), cadmium (MIC = 4 mM), and nickel (MIC = 2.5 mM) in complex medium. The MICs of these metals were more inhibitory (MIC < 2 mM) in the other tested media. The archaeal strain revealed a high sensitivity for copper and zinc, with MICs below 0.5 mM for both metals. Growth kinetics in complex and minimal media showed the strain to be more sensitive to the metals in liquid media than in solid media. The growth kinetic assays indicated the presence of selected heavy metals resulted in a lower growth rate and lower total cell mass relative to the control. Despite that cadmium and lead are nonessential and have no nutrient value, they were the most tolerated metals by H. salinarum strain. In addition, pigment intensity in the strain was inhibited by the presence of the heavy metals relative to the control.

Citing Articles

Elucidating metabolic pathways through genomic analysis in highly heavy metal-resistant strains.

Baati H, Siala M, Benali S, Azri C, Dunlap C, Martinez-Espinosa R Heliyon. 2024; 10(23):e40822.

PMID: 39717611 PMC: 11665356. DOI: 10.1016/j.heliyon.2024.e40822.

Arsenite tolerance and removal potential of the indigenous halophilic bacterium, Halomonas elongata SEK2.

Tavoosi N, Sepahi A, Kiarostami V, Amoozegar M Biometals. 2024; 37(6):1393-1409.

PMID: 38822902 DOI: 10.1007/s10534-024-00612-2.

Screening of heavy metal-resistant rhizobial and non-rhizobial microflora isolated from Trifolium sp. growing in mining areas.

Rahal S, Menaa B, Chekireb D Environ Monit Assess. 2024; 196(3):283.

PMID: 38372826 DOI: 10.1007/s10661-024-12445-0.

Hydrolytic enzyme screening and carotenoid production evaluation of halophilic archaea isolated from highly heavy metal-enriched solar saltern sediments.

Baati H, Siala M, Azri C, Ammar E, Trigui M Braz J Microbiol. 2022; 53(4):1893-1906.

PMID: 36280648 PMC: 9679124. DOI: 10.1007/s42770-022-00855-6.

Genomic analysis of heavy metal-resistant Halobacterium salinarum isolated from Sfax solar saltern sediments.

Baati H, Siala M, Azri C, Ammar E, Dunlap C, Trigui M Extremophiles. 2022; 26(2):25.

PMID: 35842547 PMC: 9288257. DOI: 10.1007/s00792-022-01273-0.

References

Al-Momani F, Massadeh A, Hadad Y . Uptake of zinc and copper by halophilic bacteria isolated from the Dead Sea shore, Jordan. Biol Trace Elem Res. 2007; 115(3):291-300. DOI: 10.1007/BF02686003. View

Andreini C, Banci L, Bertini I, Rosato A . Zinc through the three domains of life. J Proteome Res. 2006; 5(11):3173-8. DOI: 10.1021/pr0603699. View

Baati H, Guermazi S, Amdouni R, Gharsallah N, Sghir A, Ammar E . Prokaryotic diversity of a Tunisian multipond solar saltern. Extremophiles. 2008; 12(4):505-18. DOI: 10.1007/s00792-008-0154-x. View

Berney M, Weilenmann H, Ihssen J, Bassin C, Egli T . Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection. Appl Environ Microbiol. 2006; 72(4):2586-93. PMC: 1449012. DOI: 10.1128/AEM.72.4.2586-2593.2006. View

Bini E . Archaeal transformation of metals in the environment. FEMS Microbiol Ecol. 2010; 73(1):1-16. DOI: 10.1111/j.1574-6941.2010.00876.x. View

Boujelben I, Martinez-Garcia M, van Pelt J, Maalej S . Diversity of cultivable halophilic archaea and bacteria from superficial hypersaline sediments of Tunisian solar salterns. Antonie Van Leeuwenhoek. 2014; 106(4):675-92. DOI: 10.1007/s10482-014-0238-9. View

Chasapis C, Andreini C, Georgiopolou A, Stefanidou M, Vlamis-Gardikas A . Identification of the zinc, copper and cadmium metalloproteome of the protozoon Tetrahymena thermophila by systematic bioinformatics. Arch Microbiol. 2017; 199(8):1141-1149. DOI: 10.1007/s00203-017-1385-y. View

Dammak R, Bahloul M, Chabbi I, Azri C . Spatial and temporal variations of dust particle deposition at three "urban/suburban" areas in Sfax city (Tunisia). Environ Monit Assess. 2016; 188(6):336. DOI: 10.1007/s10661-016-5341-0. View

Das D, Salgaonkar B, Mani K, Braganca J . Cadmium resistance in extremely halophilic archaeon Haloferax strain BBK2. Chemosphere. 2014; 112:385-92. DOI: 10.1016/j.chemosphere.2014.04.058. View

10.

Huo Y, Li Z, Cheng H, Wang C, Xu X . High quality draft genome sequence of the heavy metal resistant bacterium Halomonas zincidurans type strain B6(T). Stand Genomic Sci. 2015; 9:30. PMC: 4286145. DOI: 10.1186/1944-3277-9-30. View

11.

Kaur A, Pan M, Meislin M, Facciotti M, El-Gewely R, Baliga N . A systems view of haloarchaeal strategies to withstand stress from transition metals. Genome Res. 2006; 16(7):841-54. PMC: 1484451. DOI: 10.1101/gr.5189606. View

12.

Kottemann M, Kish A, Iloanusi C, Bjork S, DiRuggiero J . Physiological responses of the halophilic archaeon Halobacterium sp. strain NRC1 to desiccation and gamma irradiation. Extremophiles. 2005; 9(3):219-27. DOI: 10.1007/s00792-005-0437-4. View

13.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

14.

Macomber L, Hausinger R . Mechanisms of nickel toxicity in microorganisms. Metallomics. 2011; 3(11):1153-62. PMC: 4130172. DOI: 10.1039/c1mt00063b. View

15.

Mani K, Salgaonkar B, Braganca J . Culturable halophilic archaea at the initial and crystallization stages of salt production in a natural solar saltern of Goa, India. Aquat Biosyst. 2012; 8(1):15. PMC: 3444409. DOI: 10.1186/2046-9063-8-15. View

16.

Moreno M, Piubeli F, Bonfa M, Garcia M, Durrant L, Mellado E . Analysis and characterization of cultivable extremophilic hydrolytic bacterial community in heavy-metal-contaminated soils from the Atacama Desert and their biotechnological potentials. J Appl Microbiol. 2012; 113(3):550-9. DOI: 10.1111/j.1365-2672.2012.05366.x. View

17.

Nieto J, Ventosa A, Ruiz-Berraquero F . Susceptibility of halobacteria to heavy metals. Appl Environ Microbiol. 1987; 53(5):1199-202. PMC: 203836. DOI: 10.1128/aem.53.5.1199-1202.1987. View

18.

Oren A . Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. Int J Syst Evol Microbiol. 2011; 62(Pt 2):263-271. DOI: 10.1099/ijs.0.038653-0. View

19.

Raghavan T, Furtado I . Expression of carotenoid pigments of haloarchaeal cultures exposed to aniline. Environ Toxicol. 2005; 20(2):165-9. DOI: 10.1002/tox.20091. View

20.

Rios M, Nieto J, Ventosa A . Numerical taxonomy of heavy metal-tolerant nonhalophilic bacteria isolated from hypersaline environments. Int Microbiol. 2000; 1(1):45-51. View