A Novel Platform Using RNA Signatures To Accelerate Antimicrobial Susceptibility Testing in Neisseria Gonorrhoeae

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2020 Sep 24

PMID 32967905

Citations 5

Authors

Marjan M Hashemi

Nikhil Ram-Mohan

Xi Yang

Nadya Andini

Nicholas R Gessner

Karen C Carroll

Tza-Huei Wang

Samuel Yang

Affiliations

Soon will be listed here.

Abstract

The rise of antimicrobial-resistant pathogens can be attributed to the lack of a rapid pathogen identification (ID) or antimicrobial susceptibility testing (AST), resulting in delayed therapeutic decisions at the point of care. Gonorrhea is usually empirically treated, with no AST results available before treatment, thus contributing to the rapid rise in drug resistance. Here, we present a rapid AST platform using RNA signatures for Transcriptome sequencing (RNA-seq) followed by bioinformatic tools was applied to explore potential markers in the transcriptome profile of upon minutes of azithromycin exposure. Validation of candidate markers using quantitative real-time PCR (qRT-PCR) showed that two markers ( [NGO1562] and ) can deliver accurate AST results across 14 tested isolates. Further validation of our susceptibility threshold in comparison to MIC across 64 more isolates confirmed the reliability of our platform. Our RNA markers combined with emerging molecular point-of-care systems has the potential to greatly accelerate both ID and AST to inform treatment.

Citing Articles

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