Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite-Silica-Glass Ionomer Cement

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2020 Sep 22

PMID 32957636

Citations 5

Authors

Hii Siew Ching

Kannan Thirumulu Ponnuraj

Norhayati Luddin

Ismail Ab Rahman

Nik Rozainah Nik Abdul Ghani

Affiliations

Soon will be listed here.

Abstract

This study aimed to investigate the effects of nanohydroxyapatite-silica-glass ionomer cement (nanoHA-silica-GIC) on the differentiation of dental pulp stem cells (DPSCs) into odontogenic lineage. DPSCs were cultured in complete Minimum Essential Medium Eagle-Alpha Modification (-MEM) with or without nanoHA-silica-GIC extract and conventional glass ionomer cement (cGIC) extract. Odontogenic differentiation of DPSCs was evaluated by real-time reverse transcription polymerase chain reaction (rRT-PCR) for odontogenic markers: dentin sialophosphoprotein (), dentin matrix protein 1 (), osteocalcin (), osteopontin (), alkaline phosphatase (), collagen type I (), and runt-related transcription factor 2 () on day 1, 7, 10, 14, and 21, which were normalized to the house keeping gene glyceraldehyde-3-phosphate dehydrogenase (). Untreated DPSCs were used as a control throughout the study. The expressions of and were higher on days 7 and 10, that of on day 10, those of and on day 14, and that of on day 1; exhibited a time-dependent increase from day 7 to day 14. Despite the above time-dependent variations, the expressions were comparable at a concentration of 6.25 mg/mL between the nanoHA-silica-GIC and cGIC groups. This offers empirical support that nanoHA-silica-GIC plays a role in the odontogenic differentiation of DPSCs.

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