Calcium Supplementation Shows a Hepatoprotective Effect Against High-fat Diet by Regulating Oxidative-induced Inflammatory Response and Lipogenesis Activity in Male Rats

Overview

Journal J Tradit Complement Med

Specialty Rehabilitation Medicine

Date 2020 Sep 21

PMID 32953568

Citations 6

Authors

Sandeep Das

Dipayan Choudhuri

Affiliations

Soon will be listed here.

Abstract

Background And Aim: High-fat diet (HFD) triggers obesity-related metabolic diseases like non-alcoholic fatty liver diseases (NAFLD). Calcium supplementation is known to have an anti-obesity effect. However, the effect of calcium supplementation has not been evaluated so far in context to hepatic functions on exposure to HFD. The goal of the present study was to investigate the role of calcium supplementation on hepatic function and other physiological markers in HFD induced NAFLD rats.

Experimental Procedure: 18 male Wistar rats were divided into two groups; first group considered control group (n = 6) for the entire treatment period and the second group (n = 12) fed with HFD for 6 weeks to induce NAFLD model and then sub-divided into two groups (n = 6 rats); one group received HFD and other group received 1.0 gm CaCO/100 gm HFD for 30 days. After treatment, all animals were euthanized to collect the blood and liver for biochemical, enzymatic, oxidative, anti-oxidant, western blot and histological study.

Results And Conclusion: Calcium supplementation significantly improved the anthropometric parameters and decreases the level of serum cholesterol, triglyceride, FFA and hepatic enzymes. Calcium supplementation significantly down-regulated the hepatic PPAR-γ mediated FAS activity, hepatic lipid accumulation, oxidative stress and restored the activities of antioxidant enzyme which further prevented the stimulation of pro-inflammatory response. Calcium supplementation also increases the hepatic protein expression of phosphorylated AMP-activated protein kinase. So, calcium supplementation showed a hepatoprotective effect during NAFLD by downregulating the oxidative induced inflammatory response stimulated by hepatic lipogenesis activity and subsequent lipid accumulation.

Citing Articles

Role of Leaf Phytosomes Against Oxidative Stress and Inflammation in Rats Fed with a High-Fat-Fructose Diet.

Ortega-Perez L, Hernandez-Soto J, Padilla-Avalos O, Ayala-Ruiz L, Magana-Rodriguez O, Pinon-Simental J Antioxidants (Basel). 2024; 13(10).

PMID: 39456515 PMC: 11504497. DOI: 10.3390/antiox13101263.

Anti-obesity effects and underlying molecular mechanisms of the ethanolic extract of figs from Ficus hispida using high fat-fed wister rats.

Shama A, Shova L, Bristy A, Emran T, Shabnam S, Shill M Heliyon. 2024; 10(15):e35392.

PMID: 39170114 PMC: 11336639. DOI: 10.1016/j.heliyon.2024.e35392.

Calcium, Phosphate, and Vitamin D in Children and Adolescents with Chronic Diseases: A Cross-Sectional Study.

Escobedo-Monge M, Bahillo-Curieses P, Parodi-Roman J, Escobedo-Monge M, Alonso-Lopez P, Marugan-Miguelsanz J Nutrients. 2024; 16(9).

PMID: 38732596 PMC: 11085162. DOI: 10.3390/nu16091349.

The effects of E'Jiao on body composition, bone marrow adiposity and skeletal redox status in ovariectomised rats.

Ekeuku S, Chin K, Qian J, Zhang Y, Qu H, Ahmad F Int J Med Sci. 2023; 20(13):1711-1721.

PMID: 37928881 PMC: 10620870. DOI: 10.7150/ijms.84604.

Jaroenlapnopparat A, Suppakitjanusant P, Ponvilawan B, Charoenngam N Int J Mol Sci. 2022; 23(16).

PMID: 36012386 PMC: 9409408. DOI: 10.3390/ijms23169122.

References

Zemel M . Mechanisms of dairy modulation of adiposity. J Nutr. 2003; 133(1):252S-256S. DOI: 10.1093/jn/133.1.252S. View

He Y, Li S, Wang Y, Wang G, He Y, Liao X . Postweaning low-calcium diet promotes later-life obesity induced by a high-fat diet. J Nutr Biochem. 2012; 23(10):1238-44. DOI: 10.1016/j.jnutbio.2011.07.004. View

Poudyal H, Campbell F, Brown L . Olive leaf extract attenuates cardiac, hepatic, and metabolic changes in high carbohydrate-, high fat-fed rats. J Nutr. 2010; 140(5):946-53. DOI: 10.3945/jn.109.117812. View

Steinberg G, Kemp B . AMPK in Health and Disease. Physiol Rev. 2009; 89(3):1025-78. DOI: 10.1152/physrev.00011.2008. View

Green L, Wagner D, Glogowski J, Skipper P, Wishnok J, Tannenbaum S . Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem. 1982; 126(1):131-8. DOI: 10.1016/0003-2697(82)90118-x. View

Reznick A, Packer L . Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol. 1994; 233:357-63. DOI: 10.1016/s0076-6879(94)33041-7. View

Christensen R, Lorenzen J, Svith C, Bartels E, Melanson E, Saris W . Effect of calcium from dairy and dietary supplements on faecal fat excretion: a meta-analysis of randomized controlled trials. Obes Rev. 2009; 10(4):475-86. DOI: 10.1111/j.1467-789X.2009.00599.x. View

Sasidharan S, Joseph J, Anandakumar S, Venkatesan V, Madhavan C, Agarwal A . Ameliorative potential of Tamarindus indica on high fat diet induced nonalcoholic fatty liver disease in rats. ScientificWorldJournal. 2014; 2014:507197. PMC: 3932208. DOI: 10.1155/2014/507197. View

Noeman S, Hamooda H, Baalash A . Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol Metab Syndr. 2011; 3(1):17. PMC: 3174870. DOI: 10.1186/1758-5996-3-17. View

10.

Vidal-Puig A, Jimenez-Linan M, Lowell B, Hamann A, Hu E, Spiegelman B . Regulation of PPAR gamma gene expression by nutrition and obesity in rodents. J Clin Invest. 1996; 97(11):2553-61. PMC: 507341. DOI: 10.1172/JCI118703. View

11.

Smith B, Marcinko K, Desjardins E, Lally J, Ford R, Steinberg G . Treatment of nonalcoholic fatty liver disease: role of AMPK. Am J Physiol Endocrinol Metab. 2016; 311(4):E730-E740. DOI: 10.1152/ajpendo.00225.2016. View

12.

Meli R, Raso G, Calignano A . Role of innate immune response in non-alcoholic Fatty liver disease: metabolic complications and therapeutic tools. Front Immunol. 2014; 5:177. PMC: 4005965. DOI: 10.3389/fimmu.2014.00177. View

13.

Guilherme A, Tesz G, Guntur K, Czech M . Tumor necrosis factor-alpha induces caspase-mediated cleavage of peroxisome proliferator-activated receptor gamma in adipocytes. J Biol Chem. 2009; 284(25):17082-17091. PMC: 2719346. DOI: 10.1074/jbc.M809042200. View

14.

Than N, Newsome P . A concise review of non-alcoholic fatty liver disease. Atherosclerosis. 2015; 239(1):192-202. DOI: 10.1016/j.atherosclerosis.2015.01.001. View

15.

Ellman G . Tissue sulfhydryl groups. Arch Biochem Biophys. 1959; 82(1):70-7. DOI: 10.1016/0003-9861(59)90090-6. View

16.

Wan S, Zhang L, Quan Y, Wei K . Resveratrol-loaded PLGA nanoparticles: enhanced stability, solubility and bioactivity of resveratrol for non-alcoholic fatty liver disease therapy. R Soc Open Sci. 2018; 5(11):181457. PMC: 6281916. DOI: 10.1098/rsos.181457. View

17.

Musso G, Gambino R, Cassader M . Recent insights into hepatic lipid metabolism in non-alcoholic fatty liver disease (NAFLD). Prog Lipid Res. 2008; 48(1):1-26. DOI: 10.1016/j.plipres.2008.08.001. View

18.

Fabbrini E, Sullivan S, Klein S . Obesity and nonalcoholic fatty liver disease: biochemical, metabolic, and clinical implications. Hepatology. 2009; 51(2):679-89. PMC: 3575093. DOI: 10.1002/hep.23280. View

19.

Xavier H, Izar M, Faria Neto J, Assad M, Rocha V, Sposito A . [V Brazilian Guidelines on Dyslipidemias and Prevention of Atherosclerosis]. Arq Bras Cardiol. 2013; 101(4 Suppl 1):1-20. DOI: 10.5935/abc.2013S010. View

20.

Davoodi I, Rahimi R, Abdollahi M, Farzaei F, Farzaei M, Memariani Z . Promising effect of extract on high-fat diet-induced nonalcoholic fatty liver. J Tradit Complement Med. 2017; 7(4):508-514. PMC: 5634758. DOI: 10.1016/j.jtcme.2017.01.008. View