Molecular Characteristic of Activin Receptor IIB and Its Functions in Growth and Nutrient Regulation in

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2020 Sep 21

PMID 32953259

Citations 1

Authors

Jingan Wang

Kaijun Zhang

Xin Hou

Wucheng Yue

He Yang

Xiaowen Chen

Jun Wang

Chenghui Wang

Affiliations

Soon will be listed here.

Abstract

Activin receptor IIB is a serine/threonine-kinase receptor binding with transforming growth factor-β (TGF-β) superfamily ligands to participate in the regulation of muscle mass in vertebrates. However, its structure and function in crustaceans remain unknown. In this study, the gene in () was cloned and obtained with a 1,683 bp open reading frame, which contains the characteristic domains of TGF-β type II receptor superfamily, encoding 560 amino acids. The mRNA expression of was the highest in hepatopancreas and the lowest in muscle at each molting stage. After injection of double-stranded RNA during one molting cycle, the RNA interference (RNAi) group showed higher weight gain rate, higher specific growth rate, and lower hepatopancreas index compared with the control group. Meanwhile, the RNAi group displayed a significantly increased content of hydrolytic amino acid in both hepatopancreas and muscle. The RNAi group also displayed slightly higher contents of saturated fatty acid and monounsaturated fatty acid but significantly decreased levels of polyunsaturated fatty acid compared with the control group. After RNAi on , the mRNA expressions of five signaling pathway genes showed that and forkhead box O () were downregulated in hepatopancreas and muscle, but no significant expression differences were found in small mother against decapentaplegic () 3, and mammalian target of rapamycin. The mRNA expression s of three lipid metabolism-related genes (carnitine palmitoyltransferase 1β (), fatty acid synthase, and fatty acid elongation) were significantly downregulated in both hepatopancreas and muscle with the exception of in muscles. These results indicate that is a functionally conservative negative regulator in growth mass, and protein and lipid metabolism could be affected by inhibiting signaling in crustacean.

Citing Articles

Signaling Pathways That Regulate the Crustacean Molting Gland.

Mykles D Front Endocrinol (Lausanne). 2021; 12:674711.

PMID: 34234741 PMC: 8256442. DOI: 10.3389/fendo.2021.674711.

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