GlypNirO: An Automated Workflow for Quantitative - and -linked Glycoproteomic Data Analysis

Overview

Journal Beilstein J Org Chem

Specialty Chemistry

Date 2020 Sep 21

PMID 32952729

Citations 4

Authors

Toan K Phung

Cassandra L Pegg

Benjamin L Schulz

Affiliations

Soon will be listed here.

Abstract

Mass spectrometry glycoproteomics is rapidly maturing, allowing unprecedented insights into the diversity and functions of protein glycosylation. However, quantitative glycoproteomics remains challenging. We developed GlypNirO, an automated software pipeline which integrates the complementary outputs of Byonic and Proteome Discoverer to allow high-throughput automated quantitative glycoproteomic data analysis. The output of GlypNirO is clearly structured, allowing manual interrogation, and is also appropriate for input into diverse statistical workflows. We used GlypNirO to analyse a published plasma glycoproteome dataset and identified changes in site-specific - and -glycosylation occupancy and structure associated with hepatocellular carcinoma as putative biomarkers of disease.

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