Paramphistome Species in Water Buffaloes and Intermediate Hosts in the Kızılırmak Delta in Samsun Province, Turkey

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2020 Sep 19

PMID 32948931

Citations 3

Authors

Celil Ates

Sinasi Umur

Affiliations

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Abstract

Purpose: This study was carried out to determine the paramphistome species parasitizing water buffaloes (WBs) grazing in the Kızılırmak Delta in Samsun Province, Turkey, and the intermediate hosts of the parasites.

Methods: Between August 2016 and July 2018, abattoirs in Samsun Province were visited weekly and 139 slaughtered WBs were examined for paramphistome species. In the same period, 550 snails (300 Galba truncatula, 200 Physella (Physa) acuta and 50 Planorbis planorbis) were collected from pastures grazed by WBs during monthly sampling in the spring and autumn. Adult parasites were identified through the use of histological and molecular methods and larval stages were identified with a molecular method.

Results: Forty-five of the 139 WBs (32.4%) were infected with species of the family Paramphistomidae and a total of 4761 (mean 105.8) parasites were collected from them. The genera Paramphistomum and Calicophoron were distinguished from each other by examining the development status of the pars musculosa and the degree of lobulation of the testes in histological sections. Calicophoron daubneyi was present in all the infected animals and Paramphistomum cervi in only 3 of the same animals. There were larval forms in only 19 of the specimens identified as G. truncatula. Calicophoron daubneyi was molecularly diagnosed in 12 of the 19 infected G. truncatula and this result was confirmed by PCR and PCR-RFLP. Calicophoron daubneyi was also identified molecularly as being present in all 45 infected adult WBs and as developmental stages in 12 of 300 (4%) intermediate hosts, G. truncatula. The DNA sequences from the adult parasites in the definitive hosts and larval forms in intermediate hosts were allocated the codes MH939278 and MH939279, respectively, in GenBank.

Conclusion: For the first time in Turkey, C. daubneyi was identified molecularly and its intermediate host was identified as G. truncatula. Calicophoron daubneyi was identified as the overwhelmingly dominant paramphistome species in WBs in the Kızılırmak Delta, with P. cervi found in mixed infections in only three animals.

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References

Rangel-Ruiz L, Albores-Brahms S, Gamboa-Aguilar J . Seasonal trends of Paramphistomum cervi in Tabasco, Mexico. Vet Parasitol. 2003; 116(3):217-22. DOI: 10.1016/j.vetpar.2003.07.002. View

Zintl A, Garcia-Campos A, Trudgett A, Chryssafidis A, Talavera-Arce S, Fu Y . Bovine paramphistomes in Ireland. Vet Parasitol. 2014; 204(3-4):199-208. DOI: 10.1016/j.vetpar.2014.05.024. View

Gonzalez-Warleta M, Lladosa S, Castro-Hermida J, Martinez-Ibeas A, Conesa D, Munoz F . Bovine paramphistomosis in Galicia (Spain): prevalence, intensity, aetiology and geospatial distribution of the infection. Vet Parasitol. 2012; 191(3-4):252-63. DOI: 10.1016/j.vetpar.2012.09.006. View

Wenzel C, Kuchler A, Strube C, Knubben-Schweizer G . [Paramphistomidosis - an overview on epidemiology and clinical signs]. Tierarztl Prax Ausg G Grosstiere Nutztiere. 2019; 47(3):184-191. DOI: 10.1055/a-0880-6381. View

Fuertes M, Perez V, Benavides J, Gonzalez-Lanza M, Mezo M, Gonzalez-Warleta M . Pathological changes in cattle naturally infected by Calicophoron daubneyi adult flukes. Vet Parasitol. 2015; 209(3-4):188-96. DOI: 10.1016/j.vetpar.2015.02.034. View

Sanabria R, Moreno L, Alvarez L, Lanusse C, Romero J . Efficacy of oxyclozanide against adult Paramphistomum leydeni in naturally infected sheep. Vet Parasitol. 2014; 206(3-4):277-81. DOI: 10.1016/j.vetpar.2014.09.022. View

Jones R, Williams H, Dalesman S, Ayodeji S, Thomas R, Brophy P . The prevalence and development of digenean parasites within their intermediate snail host, Galba truncatula, in a geographic area where the presence of Calicophoron daubneyi has recently been confirmed. Vet Parasitol. 2017; 240:68-74. PMC: 5469397. DOI: 10.1016/j.vetpar.2017.03.021. View

Martinez-Ibeas A, Gonzalez-Warleta M, Martinez-Valladares M, Castro-Hermida J, Gonzalez-Lanza C, Minambres B . Development and validation of a mtDNA multiplex PCR for identification and discrimination of Calicophoron daubneyi and Fasciola hepatica in the Galba truncatula snail. Vet Parasitol. 2013; 195(1-2):57-64. DOI: 10.1016/j.vetpar.2012.12.048. View

Sanabria R, More G, Romero J . Molecular characterization of the ITS-2 fragment of Paramphistomum leydeni (Trematoda: Paramphistomidae). Vet Parasitol. 2010; 177(1-2):182-5. DOI: 10.1016/j.vetpar.2010.11.023. View

10.

Sindicic M, Martinkovic F, Striskovic T, Spehar M, Stimac I, Bujanic M . Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus. J Helminthol. 2016; 91(5):637-641. DOI: 10.1017/S0022149X16000699. View

11.

Itagaki T, Tsumagari N, Tsutsumi K, Chinone S . Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers. J Vet Med Sci. 2003; 65(8):931-3. DOI: 10.1292/jvms.65.931. View

12.

Rinaldi L, Perugini A, Capuano F, Fenizia D, Musella V, Veneziano V . Characterization of the second internal transcribed spacer of ribosomal DNA of Calicophoron daubneyi from various hosts and locations in southern Italy. Vet Parasitol. 2005; 131(3-4):247-53. DOI: 10.1016/j.vetpar.2005.04.035. View

13.

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

14.

Samnaliev P, Pino L, Bayssade-Dufour C, Albaret J . [Superficial argentophilic structures of miracidia and cercaria of Paramphistomum leydeni Nasmark 1937]. Ann Parasitol Hum Comp. 1984; 59(2):151-9. View

15.

Ferreras M, Gonzalez-Lanza C, Perez V, Fuertes M, Benavides J, Mezo M . Calicophoron daubneyi (Paramphistomidae) in slaughtered cattle in Castilla y León (Spain). Vet Parasitol. 2013; 199(3-4):268-71. DOI: 10.1016/j.vetpar.2013.10.019. View