Phylogenetic Diversity in and Gene Clusters Between and , As a Potential Cause of Host Specificity

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2020 Sep 18

PMID 32944148

Citations 10

Authors

Kaori Fujiwara-Takahashi

Takayasu Watanabe

Masahiro Shimogishi

Masaki Shibasaki

Makoto Umeda

Yuichi Izumi

Ichiro Nakagawa

Affiliations

Soon will be listed here.

Abstract

Background: Periodontopathic bacteria in humans and in animals are phylogenetically close and commonly have FimA and Mfa1 fimbriae. However, little is known about how and are phylogenetically different between and . Here, we examined phylogenetic diversity in their and gene clusters.

Methods: Twenty strains were isolated from the periodontal pocket of 20 dogs. For their genomic information, along with 64 and 11 genomes, phylogenetic relationship between the genotypes of and was examined. Variability of amino acid sequences was examined in the three-dimensional structure of FimA. The distance between strains was calculated for and genes.

Results: Some genotypes in were close to particular types in . Two types of were classified as 70-kDa and 53-kDa protein-coding . The variable amino acid positions were primarily at the outer part of FimA. The genes encoding the structural proteins and the main component were similarly distant from the reference strain in , but not in .

Conclusions: The differences in the gene clusters between and may result in their host specificity.

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