Network Analysis of Targets Showing the Potential Oncogenic Role of in Colorectal Cancer

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2020 Sep 18

PMID 32943987

Citations 9

Authors

Qi Liao

Linbo Chen

Ning Zhang

Yang Xi

Shiyun Hu

Derry Minyao Ng

Fatma Yislam Hadi Ahmed

Guofang Zhao

Xiaoxiang Fan

Yangyang Xie

Xiaoyu Dai

Yanping Jin

Jiaxin Ge

Changzheng Dong

Xinjun Zhang

Junming Guo

Affiliations

Soon will be listed here.

Abstract

Background: is a member of the Kruppel-like factor, subfamily of zinc finger proteins that are involved in cancers. functions as a transcription factor and regulates the diverse protein-coding genes (PCGs) in colorectal cancer (CRC). However, the long non-coding RNAs (lncRNAs) regulated by in CRC are currently unknown.

Methods: In this study, we first designed a computational pipeline to determine the PCG and lncRNA targets of in CRC. Then we analyzed the motif pattern of the binding regions for the lncRNA targets. The regulatory co-factors of were then searched for through bioinformatics analysis. We also constructed a regulatory network for and annotated its functions. Finally, one of the lncRNA targets, , was selected to further explore its expression pattern and functions in CRC.

Results: We were able to identify 19 lncRNA targets of and found that the motifs of the lncRNA binding sites were GC-enriched. Next, we pinpointed the transcription factors and as the regulatory co-factors of through bioinformatics analysis. Then, through the analysis of the regulatory network, we found that may be involved in DNA replication, DNA repair, and the cell cycle. Furthermore, in the cell cycle module, the up-regulating expression pattern was verified in the CRC cell lines and tissues, associating it to CRC invasion and distal metastasis. This indicates that may play a critical part in CRC tumorigenesis and progression. Additionally, expression of was found to be down-regulated in CRC cell lines when expression was knocked-down by siRNA; and a strong correlation was observed between the expression levels of and , further alluding to their regulatory relationship.

Conclusions: In conclusion, the network analysis of targets indicates that may be a significant lncRNA in CRC.

Citing Articles

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