The Conserved Elongation Factor Spn1 is Required for Normal Transcription, Histone Modifications, and Splicing in Saccharomyces Cerevisiae

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2020 Sep 17

PMID 32941642

Citations 11

Authors

Natalia I Reim

James Chuang

Dhawal Jain

Burak H Alver

Peter J Park

Fred Winston

Affiliations

Soon will be listed here.

Abstract

Spn1/Iws1 is a conserved protein involved in transcription and chromatin dynamics, yet its general in vivo requirement for these functions is unknown. Using a Spn1 depletion system in Saccharomyces cerevisiae, we demonstrate that Spn1 broadly influences several aspects of gene expression on a genome-wide scale. We show that Spn1 is globally required for normal mRNA levels and for normal splicing of ribosomal protein transcripts. Furthermore, Spn1 maintains the localization of H3K36 and H3K4 methylation across the genome and is required for normal histone levels at highly expressed genes. Finally, we show that the association of Spn1 with the transcription machinery is strongly dependent on its binding partner, Spt6, while the association of Spt6 and Set2 with transcribed regions is partially dependent on Spn1. Taken together, our results show that Spn1 affects multiple aspects of gene expression and provide additional evidence that it functions as a histone chaperone in vivo.

Citing Articles

THE HISTONE CHAPERONE SPN1 PRESERVES CHROMATIN PROTECTIONS AT PROMOTERS AND NUCLEOSOME POSITIONING IN OPEN READING FRAMES.

Tonsager A, Zukowski A, Radebaugh C, Weirich A, Stargell L, Ramachandran S bioRxiv. 2024; .

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Insights into Spt6: a histone chaperone that functions in transcription, DNA replication, and genome stability.

Miller C, Warner J, Winston F Trends Genet. 2023; 39(11):858-872.

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Domains and residues of the Saccharomyces cerevisiae hnRNP protein Hrp1 important for transcriptional autoregulation and noncoding RNA termination.

Goguen E, Brow D Genetics. 2023; 225(1).

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Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy at active genes in Saccharomyces cerevisiae.

Ellison M, Namjilsuren S, Shirra M, Blacksmith M, Schusteff R, Kerr E Nucleic Acids Res. 2023; 51(10):4814-4830.

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