Using Telomeric Chromosomal Aberrations to Evaluate Clastogen-induced Genomic Instability in Mammalian Cells

Overview

Journal Chromosome Res

Date 2020 Sep 17

PMID 32940874

Citations 5

Authors

Alejandro D Bolzan

Affiliations

Soon will be listed here.

Abstract

Telomeres, the specialized nucleoproteic complexes localized at the physical ends of linear eukaryotic chromosomes, play a fundamental role in maintaining chromosomal stability and integrity, being one of the leading guardians of genome stability. In recent years, the identification and analysis of chromosomal aberrations involving telomeres has proven to be a unique tool to evaluate misrepaired and unrepaired chromosome damage in mammalian cells. Telomere instability constitutes an important source of genomic instability, a phenomenon characteristic of cancer cells, and also common in cells exposed to chemical or physical mutagens which induce chromosomal aberrations by producing chromosome breakage (clastogens). In the present review, we will focus on the chromosomal aberrations involving telomeres and their importance to determine the clastogen-induced genomic instability present in mammalian cells.

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References

Ahmed W, Lingner J . Impact of oxidative stress on telomere biology. Differentiation. 2017; 99:21-27. DOI: 10.1016/j.diff.2017.12.002. View

Al-Wahiby S, Slijepcevic P . Chromosomal aberrations involving telomeres in BRCA1 deficient human and mouse cell lines. Cytogenet Genome Res. 2005; 109(4):491-6. DOI: 10.1159/000084208. View

Arnoult N, Karlseder J . Complex interactions between the DNA-damage response and mammalian telomeres. Nat Struct Mol Biol. 2015; 22(11):859-66. PMC: 4739752. DOI: 10.1038/nsmb.3092. View

Artandi S, DePinho R . Telomeres and telomerase in cancer. Carcinogenesis. 2009; 31(1):9-18. PMC: 3003493. DOI: 10.1093/carcin/bgp268. View

Ayouaz A, Raynaud C, Heride C, Revaud D, Sabatier L . Telomeres: hallmarks of radiosensitivity. Biochimie. 2007; 90(1):60-72. DOI: 10.1016/j.biochi.2007.09.011. View

Azzalin C, Lingner J . Telomeres: the silence is broken. Cell Cycle. 2008; 7(9):1161-5. DOI: 10.4161/cc.7.9.5836. View

Bailey S, Cornforth M, Ullrich R, Goodwin E . Dysfunctional mammalian telomeres join with DNA double-strand breaks. DNA Repair (Amst). 2004; 3(4):349-57. DOI: 10.1016/j.dnarep.2003.11.007. View

Bailey S, Goodwin E, Cornforth M . Strand-specific fluorescence in situ hybridization: the CO-FISH family. Cytogenet Genome Res. 2004; 107(1-2):14-7. DOI: 10.1159/000079565. View

Barnes R, Fouquerel E, Opresko P . The impact of oxidative DNA damage and stress on telomere homeostasis. Mech Ageing Dev. 2018; 177:37-45. PMC: 6162185. DOI: 10.1016/j.mad.2018.03.013. View

10.

Bejarano L, Bosso G, Louzame J, Serrano R, Gomez-Casero E, Martinez-Torrecuadrada J . Multiple cancer pathways regulate telomere protection. EMBO Mol Med. 2019; 11(7):e10292. PMC: 6609915. DOI: 10.15252/emmm.201910292. View

11.

Benkhaled L, Barrios L, Mestres M, Caballin M, Ribas M, Barquinero J . Analysis of gamma-rays induced chromosome aberrations: a fingerprint evaluation with a combination of pan-centromeric and pan-telomeric probes. Int J Radiat Biol. 2006; 82(12):869-75. DOI: 10.1080/09553000600979092. View

12.

Benkhaled L, Xuncla M, Caballin M, Barrios L, Barquinero J . Induction of complete and incomplete chromosome aberrations by bleomycin in human lymphocytes. Mutat Res. 2007; 637(1-2):134-41. DOI: 10.1016/j.mrfmmm.2007.07.013. View

13.

Berardinelli F, Antoccia A, Cherubini R, De Nadal V, Gerardi S, Cirrone G . Transient activation of the ALT pathway in human primary fibroblasts exposed to high-LET radiation. Radiat Res. 2010; 174(5):539-49. DOI: 10.1667/RR2127.1. View

14.

Berardinelli F, Antoccia A, Buonsante R, Gerardi S, Cherubini R, De Nadal V . The role of telomere length modulation in delayed chromosome instability induced by ionizing radiation in human primary fibroblasts. Environ Mol Mutagen. 2013; 54(3):172-9. DOI: 10.1002/em.21761. View

15.

Berardinelli F, Sgura A, Di Masi A, Leone S, Cirrone G, Romano F . Radiation-induced telomere length variations in normal and in Nijmegen Breakage Syndrome cells. Int J Radiat Biol. 2013; 90(1):45-52. DOI: 10.3109/09553002.2014.859400. View

16.

Berardinelli F, Coluzzi E, Sgura A, Antoccia A . Targeting telomerase and telomeres to enhance ionizing radiation effects in in vitro and in vivo cancer models. Mutat Res Rev Mutat Res. 2017; 773:204-219. DOI: 10.1016/j.mrrev.2017.02.004. View

17.

Bhargava R, Fischer M, OSullivan R . Genome rearrangements associated with aberrant telomere maintenance. Curr Opin Genet Dev. 2020; 60:31-40. PMC: 7229999. DOI: 10.1016/j.gde.2020.02.005. View

18.

Blackburn E . Telomeres and telomerase: their mechanisms of action and the effects of altering their functions. FEBS Lett. 2005; 579(4):859-62. DOI: 10.1016/j.febslet.2004.11.036. View

19.

Boei J, Natarajan A . Combined use of chromosome painting and telomere detection to analyse radiation-induced chromosomal aberrations in mouse splenocytes. Int J Radiat Biol. 1998; 73(2):125-33. DOI: 10.1080/095530098142491. View

20.

Boei J, Vermeulen S, Fomina J, Natarajan A . Detection of incomplete exchanges and interstitial fragments in X-irradiated human lymphocytes using a telomeric PNA probe. Int J Radiat Biol. 1998; 73(6):599-603. DOI: 10.1080/095530098141843. View