An Amperometric Polyphenol Biosensor Based on Laccase Immobilized on Epoxy Resin Membrane

Overview

Journal Anal Methods

Specialties Biochemistry
Chemistry

Date 2020 Sep 17

PMID 32938095

Citations 9

Authors

Sheetal Chawla

Rachna Rawal

Shabnam

R C Kuhad

C S Pundir

Affiliations

Soon will be listed here.

Abstract

A polyphenol biosensor employing epoxy resin membrane bound Ganoderma sp. laccase has been developed. The biosensor showed optimum response within 30 s, when operated at 0.4 V in 0.1 M acetate buffer, pH 6.0 and 35 °C. Detection limit of the biosensor was 3.0 x 10-7 M. Analytical recovery of added guaiacol was 96.66%. Within batch and between batch coefficients of variation were <1.35% and <2.97% respectively. The biosensor was employed for amperometric determination of polyphenols in fruit juices and alcoholic beverages. The enzyme electrode was used 200 times over a period of 10 months, when stored at 4 °C.

Citing Articles

Applications and immobilization strategies of the copper-centred laccase enzyme; a review.

Kyomuhimbo H, Brink H Heliyon. 2023; 9(2):e13156.

PMID: 36747551 PMC: 9898315. DOI: 10.1016/j.heliyon.2023.e13156.

Progress in the Development of Intrinsically Conducting Polymer Composites as Biosensors.

Prajapati D, Kandasubramanian B Macromol Chem Phys. 2020; 220(10):1800561.

PMID: 32327916 PMC: 7168478. DOI: 10.1002/macp.201800561.

Spectrophotometric and Smartphone-Assisted Determination of Phenolic Compounds Using Crude Eggplant Extract.

Morosanova M, Bashkatova A, Morosanova E Molecules. 2019; 24(23).

PMID: 31810325 PMC: 6930448. DOI: 10.3390/molecules24234407.

Screening and production of a potential extracellular fungal laccase from : Media optimization by response surface methodology (RSM) and central composite rotatable design (CCRD).

Senthivelan T, Kanagaraj J, Panda R, Narayani T Biotechnol Rep (Amst). 2019; 23:e00344.

PMID: 31193735 PMC: 6541886. DOI: 10.1016/j.btre.2019.e00344.

Electrochemical Study and Characterization of an Amperometric Biosensor Based on the Immobilization of Laccase in a Nanostructure of TiO₂ Synthesized by the Sol-Gel Method.

Romero-Arcos M, Garnica-Romo M, Martinez-Flores H Materials (Basel). 2017; 9(7).

PMID: 28773666 PMC: 5456923. DOI: 10.3390/ma9070543.