Apparent Prevalence and Risk Factors of Coxiellosis (Q Fever) Among Dairy Herds in India

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Sep 15

PMID 32931511

Citations 17

Authors

Pankaj Dhaka

Satya Veer Singh Malik

Jay Prakash Yadav

Manesh Kumar

Sukhadeo B Barbuddhe

Deepak B Rawool

Affiliations

Soon will be listed here.

Abstract

Coxiella burnetii is a highly infectious zoonotic pathogen infecting wide range of mammals, including humans. In the present study, a total of 711 blood samples from bovines [cattle (n = 543) and buffaloes (n = 168)] from eight farms at different geographical locations in India were screened for C. burnetii targeting the IS1111 and the com1 genes. The anti-C. burnetii antibodies in serum samples were detected using indirect-ELISA kits. Also, a total of 21 parameters pertaining to animal health and farm management were identified to assess their role as possible risk factors for coxiellosis among the targeted farms. The apparent prevalence (positive for PCR and/or ELISA) for coxiellosis was reported to be 24.5% in cattle and 8.9% in buffaloes. In cattle, the detection rate of C. burnetii employing the IS1111 gene (8.5%) was found to be significantly higher (p<0.05) as compared to the com1 (6.5%) gene. The seropositivity by ELISA was higher among cattle (17.7%) than in buffaloes (8.3%). Further, on univariable analysis of risk factors, species (cattle) (OR:3.31; 95%CI:1.88-5.82), inadequate floor spacing (OR:1.64; 95%CI:1.10-2.43), mastitis (OR:2.35, 95%CI:1.45-3.81) and reproductive disorders (OR:2.54; 95%CI:1.67-3.85) were significantly (p<0.05) having high odds for coxiellosis. The multivariable logistic regression analysis of the animal level risk factors revealed that species and age were found to be significantly associated with coxiellosis. However, since the number of screened farms is limited; further research is needed with a higher number of animals to confirm the farm level odds ratio of risk factors. Quarantine and biosecurity measures including farm hygiene operations were observed to be inadequate and also the lack of awareness about coxiellosis among the farm workers. In absence of vaccination program for coxiellosis in India, robust surveillance, farm biosecurity measures and the awareness for the disease among risk groups can play an important role in the disease prevention and subsequent transmission of the pathogen.

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References

Candela M, Caballol A, Atance P . Wide exposure to Coxiella burnetii in ruminant and feline species living in a natural environment: zoonoses in a human-livestock-wildlife interface. Epidemiol Infect. 2016; 145(3):478-481. PMC: 9507642. DOI: 10.1017/S0950268816002454. View

Arricau Bouvery N, Souriau A, Lechopier P, Rodolakis A . Experimental Coxiella burnetii infection in pregnant goats: excretion routes. Vet Res. 2003; 34(4):423-33. DOI: 10.1051/vetres:2003017. View

Vaidya V, Malik S, Bhilegaonkar K, Rathore R, Kaur S, Barbuddhe S . Prevalence of Q fever in domestic animals with reproductive disorders. Comp Immunol Microbiol Infect Dis. 2008; 33(4):307-21. DOI: 10.1016/j.cimid.2008.10.006. View

Porter S, Czaplicki G, Mainil J, Guatteo R, Saegerman C . Q Fever: current state of knowledge and perspectives of research of a neglected zoonosis. Int J Microbiol. 2011; 2011:248418. PMC: 3238387. DOI: 10.1155/2011/248418. View

Keshavamurthy R, Singh B, Kalambhe D, Aulakh R, Dhand N . Prevalence of Coxiella burnetii in cattle and buffalo populations in Punjab, India. Prev Vet Med. 2019; 166:16-20. DOI: 10.1016/j.prevetmed.2019.03.003. View

Agger J, Paul S, Christoffersen A, Agerholm J . Risk factors for Coxiella burnetii antibodies in bulk tank milk from Danish dairy herds. Acta Vet Scand. 2013; 55:80. PMC: 4176746. DOI: 10.1186/1751-0147-55-80. View

Barlow J, Rauch B, Welcome F, Kim S, Dubovi E, Schukken Y . Association between Coxiella burnetii shedding in milk and subclinical mastitis in dairy cattle. Vet Res. 2008; 39(3):23. DOI: 10.1051/vetres:2007060. View

Barkallah M, Gharbi Y, Hmani M, Mallek Z, Gautier M, Gdoura R . Serological and molecular evidence of coxiellosis and risk factors in sheep flocks in central-eastern Tunisia. Comp Immunol Microbiol Infect Dis. 2018; 57:15-21. DOI: 10.1016/j.cimid.2018.02.001. View

Nokhodian Z, Ataei B, Moradi A, Yaran M, Hoseini S, Feizi A . Seroprevalence and risk factors of Coxiella burnetii infection among high-risk population in center of Iran, a neglected health problem. Acta Trop. 2017; 169:107-111. DOI: 10.1016/j.actatropica.2017.02.002. View

10.

Freick M, Enbergs H, Walraph J, Diller R, Weber J, Konrath A . Coxiella burnetii: Serological reactions and bacterial shedding in primiparous dairy cows in an endemically infected herd-impact on milk yield and fertility. Reprod Domest Anim. 2016; 52(1):160-169. DOI: 10.1111/rda.12878. View

11.

Schneeberger P, Hermans M, van Hannen E, Schellekens J, Leenders A, Wever P . Real-time PCR with serum samples is indispensable for early diagnosis of acute Q fever. Clin Vaccine Immunol. 2009; 17(2):286-90. PMC: 2815520. DOI: 10.1128/CVI.00454-09. View

12.

Menadi S, Mura A, Santucciu C, Ghalmi F, Hafsi F, Masala G . Seroprevalence and risk factors of Coxiella burnetii infection in cattle in northeast Algeria. Trop Anim Health Prod. 2019; 52(3):935-942. DOI: 10.1007/s11250-019-02083-x. View

13.

Schimmer B, Lenferink A, Schneeberger P, Aangenend H, Vellema P, Hautvast J . Seroprevalence and risk factors for Coxiella burnetii (Q fever) seropositivity in dairy goat farmers' households in The Netherlands, 2009-2010. PLoS One. 2012; 7(7):e42364. PMC: 3407076. DOI: 10.1371/journal.pone.0042364. View

14.

Galluzzo P, Villari S, Geraci F, Sciacca C, Grippi F, Curro V . Seroprevalence of Coxiella burnetii in dairy cattle from Sicily. Vet Ital. 2019; 55(3):247-252. DOI: 10.12834/VetIt.1391.7602.2. View

15.

Shome R, Deka R, Milesh L, Sahay S, Grace D, Lindahl J . Coxiella seroprevalence and risk factors in large ruminants in Bihar and Assam, India. Acta Trop. 2019; 194:41-46. DOI: 10.1016/j.actatropica.2019.03.022. View

16.

Paul S, Agger J, Markussen B, Christoffersen A, Agerholm J . Factors associated with Coxiella burnetii antibody positivity in Danish dairy cows. Prev Vet Med. 2012; 107(1-2):57-64. DOI: 10.1016/j.prevetmed.2012.05.015. View

17.

Dean A, Bonfoh B, Kulo A, Boukaya G, Amidou M, Hattendorf J . Epidemiology of brucellosis and q Fever in linked human and animal populations in northern togo. PLoS One. 2013; 8(8):e71501. PMC: 3741174. DOI: 10.1371/journal.pone.0071501. View

18.

Dhaka P, Malik S, Yadav J, Kumar M, Baranwal A, Barbuddhe S . Seroprevalence and molecular detection of coxiellosis among cattle and their human contacts in an organized dairy farm. J Infect Public Health. 2018; 12(2):190-194. DOI: 10.1016/j.jiph.2018.10.001. View

19.

Hermans T, Jeurissen L, Hackert V, Hoebe C . Land-applied goat manure as a source of human Q-fever in the Netherlands, 2006-2010. PLoS One. 2014; 9(5):e96607. PMC: 4008588. DOI: 10.1371/journal.pone.0096607. View

20.

Kersh G, Lambourn D, Raverty S, Fitzpatrick K, Self J, Akmajian A . Coxiella burnetii infection of marine mammals in the Pacific Northwest, 1997-2010. J Wildl Dis. 2012; 48(1):201-6. PMC: 11288310. DOI: 10.7589/0090-3558-48.1.201. View