High-level Eucaryotic in Vivo Expression of Biologically Active Measles Virus Hemagglutinin by Using an Adenovirus Type 5 Helper-free Vector System

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Aug 1

PMID 3292790

Citations 13

Authors

G Alkhatib

D J Briedis

Affiliations

Soon will be listed here.

Abstract

The entire measles virus (MV) hemagglutinin (HA)-coding region was reconstructed from cloned cDNAs and used as part of a hybrid transcription unit to replace a region of the adenovirus type 5 genome corresponding to the entire E1a transcription unit and most of the E1b transcription unit. The resulting recombinant virus was stable and able to replicate to high titers in 293 cells (which constitutively express the complementary E1a-E1b functions) in the absence of helper virus. During infection of 293 cells, the hybrid virus expressed MV HA protein which was indistinguishable from that expressed in MV-infected cells in terms of immunoreactivity, gel mobility, glycosylation, subcellular localization, and biologic activity. Infection of 293 cells with the hybrid virus led to high-level synthesis of the MV HA protein (equivalent to 65 to 130% of the level seen in MV-infected cells). At late times after high-multiplicity hybrid virus infection of HeLa and Vero cells (which do not express E1 functions), the level of HA protein synthesis was at least 35% of that seen in 293 cells. This MV-adenovirus recombinant will be useful in the study of the biologic properties of the MV HA protein and in assessment of the potential usefulness of hybrid adenoviruses as live-virus vaccine vectors.

Citing Articles

Future Prospects for the Development of Cost-Effective Adenovirus Vaccines.

Fougeroux C, Holst P Int J Mol Sci. 2017; 18(4).

PMID: 28420073 PMC: 5412272. DOI: 10.3390/ijms18040686.

Advances and future challenges in recombinant adenoviral vectored H5N1 influenza vaccines.

Zhang J Viruses. 2012; 4(11):2711-35.

PMID: 23202501 PMC: 3509669. DOI: 10.3390/v4112711.

Sublingual immunization with recombinant adenovirus encoding SARS-CoV spike protein induces systemic and mucosal immunity without redirection of the virus to the brain.

Shim B, Stadler K, Nguyen H, Yun C, Kim D, Chang J Virol J. 2012; 9:215.

PMID: 22995185 PMC: 3489719. DOI: 10.1186/1743-422X-9-215.

Adenoviruses as vaccine vectors.

Tatsis N, Ertl H Mol Ther. 2004; 10(4):616-29.

PMID: 15451446 PMC: 7106330. DOI: 10.1016/j.ymthe.2004.07.013.

Role for CCR5Delta32 protein in resistance to R5, R5X4, and X4 human immunodeficiency virus type 1 in primary CD4+ cells.

Agrawal L, Lu X, Qingwen J, Vanhorn-Ali Z, Nicolescu I, McDermott D J Virol. 2004; 78(5):2277-87.

PMID: 14963124 PMC: 369216. DOI: 10.1128/jvi.78.5.2277-2287.2004.

References

Jones N, Shenk T . Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell. 1979; 17(3):683-9. DOI: 10.1016/0092-8674(79)90275-7. View

Spriggs M, Murphy B, Prince G, Olmsted R, Collins P . Expression of the F and HN glycoproteins of human parainfluenza virus type 3 by recombinant vaccinia viruses: contributions of the individual proteins to host immunity. J Virol. 1987; 61(11):3416-23. PMC: 255937. DOI: 10.1128/JVI.61.11.3416-3423.1987. View

Thomas P . Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proc Natl Acad Sci U S A. 1980; 77(9):5201-5. PMC: 350025. DOI: 10.1073/pnas.77.9.5201. View

Togashi T, Orvell C, Vartdal F, Norrby E . Production of antibodies against measles virions by use of the mouse hybridoma technique. Arch Virol. 1981; 67(2):149-57. DOI: 10.1007/BF01318598. View

Thummel C, Tjian R, Grodzicker T . Expression of SV40 T antigen under control of adenovirus promoters. Cell. 1981; 23(3):825-36. DOI: 10.1016/0092-8674(81)90447-5. View

Briedis D, Lamb R, Choppin P . Influenza B virus RNA segment 8 codes for two nonstructural proteins. Virology. 1981; 112(2):417-25. DOI: 10.1016/0042-6822(81)90289-0. View

Nevins J . Mechanism of activation of early viral transcription by the adenovirus E1A gene product. Cell. 1981; 26(2 Pt 2):213-20. DOI: 10.1016/0092-8674(81)90304-4. View

Norrby E, Chen S, Togashi T, Shesberadaran H, Johnson K . Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Arch Virol. 1982; 71(1):1-11. DOI: 10.1007/BF01315171. View

Blok J, Air G, Laver W, Ward C, Lilley G, WOODS E . Studies on the size, chemical composition, and partial sequence of the neuraminidase (NA) from type A influenza viruses show that the N-terminal region of the NA is not processed and serves to anchor the NA in the viral membrane. Virology. 1982; 119(1):109-21. DOI: 10.1016/0042-6822(82)90069-1. View

10.

Carter M, Willcocks M, Loffler S, Ter Meulen V . Relationships between monoclonal antibody-binding sites on the measles virus haemagglutinin. J Gen Virol. 1982; 63 (Pt 1):113-20. DOI: 10.1099/0022-1317-63-1-113. View

11.

Shaw M, Lamb R, Erickson B, Briedis D, Choppin P . Complete nucleotide sequence of the neuraminidase gene of influenza B virus. Proc Natl Acad Sci U S A. 1982; 79(22):6817-21. PMC: 347224. DOI: 10.1073/pnas.79.22.6817. View

12.

Gaynor R, Berk A . Cis-acting induction of adenovirus transcription. Cell. 1983; 33(3):683-93. DOI: 10.1016/0092-8674(83)90011-9. View

13.

Berkner K, Sharp P . Generation of adenovirus by transfection of plasmids. Nucleic Acids Res. 1983; 11(17):6003-20. PMC: 326332. DOI: 10.1093/nar/11.17.6003. View

14.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

15.

Roux L, Waldvogel F . Defective interfering particles of Sendai virus modulate HN expression at the surface of infected BHK cells. Virology. 1983; 130(1):91-104. DOI: 10.1016/0042-6822(83)90120-4. View

16.

Gaynor R, Hillman D, Berk A . Adenovirus early region 1A protein activates transcription of a nonviral gene introduced into mammalian cells by infection or transfection. Proc Natl Acad Sci U S A. 1984; 81(4):1193-7. PMC: 344792. DOI: 10.1073/pnas.81.4.1193. View

17.

Logan J, Shenk T . Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection. Proc Natl Acad Sci U S A. 1984; 81(12):3655-9. PMC: 345277. DOI: 10.1073/pnas.81.12.3655. View

18.

Roux L, Beffy P, Portner A . Restriction of cell surface expression of Sendai virus hemagglutinin-neuraminidase glycoprotein correlates with its higher instability in persistently and standard plus defective interfering virus infected BHK-21 cells. Virology. 1984; 138(1):118-28. DOI: 10.1016/0042-6822(84)90152-1. View

19.

Bellini W, Englund G, Rozenblatt S, Arnheiter H, Richardson C . Measles virus P gene codes for two proteins. J Virol. 1985; 53(3):908-19. PMC: 254726. DOI: 10.1128/JVI.53.3.908-919.1985. View

20.

Hiebert S, PATERSON R, Lamb R . Hemagglutinin-neuraminidase protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts an N-terminal membrane anchor. J Virol. 1985; 54(1):1-6. PMC: 254752. DOI: 10.1128/JVI.54.1.1-6.1985. View