Antifungal Effects of Lactobacillus Acidophilus and Lactobacillus Plantarum Against Different Oral Candida Species Isolated from HIV/ AIDS Patients: an in Vitro Study

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2020 Sep 14

PMID 32922676

Citations 15

Authors

Samira Salari

Pooya Ghasemi Nejad Almani

Affiliations

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Abstract

Oropharyngeal Candidiasis (OPC) is an opportunistic fungal infection occurring in immunocompromised patients such as HIV/AIDS. The purpose of this study was to evaluate the antifungal properties of and on different species isolated from oral cavity of HIV/AIDS patients compared to Fluconazole (FLC). In this study, the antifungal effects of both cells and cell-free supernatants (CFSs) of and were investigated against different oral species by co-aggregation, agar overlay interference and broth microdilution assays, respectively. Our results showed that the highest co-aggregation ratio of the two tested Lactic acid bacteria (LAB) was observed for . Both and at cell concentrations 10 to 10 cfu/ml were able to inhibit the growth of most of the oral species, except for , and to some . In this study, MIC and MFC values for CFS of ranged from 100 to 200 µl/ml and 100 to 200 µl/ml, respectively, and MIC and MFC values for CFS of were 50 to 200 µl/ml and 50 to 200 µl/ml, respectively. The ranges of MIC and MFC for FLC were 256-1024 µg/ml and 512-2048 µg/ml, respectively. and displayed the highest and least susceptibility to CFSs of two LAB, respectively. Our findings showed that both cells and CFSs of and had antifungal effects against oral species.

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References

Koohestani M, Moradi M, Tajik H, Badali A . Effects of cell-free supernatant of LA5 and 431 against planktonic form and biofilm of . Vet Res Forum. 2019; 9(4):301-306. PMC: 6346487. DOI: 10.30466/vrf.2018.33086. View

Aminnezhad S, Kermanshahi R, Ranjbar R . Evaluation of Synergistic Interactions Between Cell-Free Supernatant of Lactobacillus Strains and Amikacin and Genetamicin Against Pseudomonas aeruginosa. Jundishapur J Microbiol. 2015; 8(4):e16592. PMC: 4449849. DOI: 10.5812/jjm.8(4)2015.16592. View

Simark-Mattsson C, Emilson C, Hakansson E, Jacobsson C, Roos K, Holm S . Lactobacillus-mediated interference of mutans streptococci in caries-free vs. caries-active subjects. Eur J Oral Sci. 2007; 115(4):308-14. DOI: 10.1111/j.1600-0722.2007.00458.x. View

Aarti C, Khusro A, Varghese R, Valan Arasu M, Agastian P, Al-Dhabi N . In vitro investigation on probiotic, anti-Candida, and antibiofilm properties of Lactobacillus pentosus strain LAP1. Arch Oral Biol. 2018; 89:99-106. DOI: 10.1016/j.archoralbio.2018.02.014. View

Barati M, Mirkalantari S, Ansari S, Salari S, Fattahi A . Determination of antimicotic susceptibility pattern of species isolated from patients with symptomatic candiduria. J Res Med Sci. 2019; 24:35. PMC: 6521611. DOI: 10.4103/jrms.JRMS_880_18. View

Chew S, Cheah Y, Seow H, Sandai D, Than L . Probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing Candida glabrata isolates. J Appl Microbiol. 2015; 118(5):1180-90. PMC: 4406132. DOI: 10.1111/jam.12772. View

Gudadappanavar A, Hombal P, Timashetti S, Javali S . Influence of and on wound healing in male Wistar rats - an experimental study. Int J Appl Basic Med Res. 2018; 7(4):233-238. PMC: 5752807. DOI: 10.4103/ijabmr.IJABMR_329_16. View

Coman M, Verdenelli M, Cecchini C, Silvi S, Orpianesi C, Boyko N . In vitro evaluation of antimicrobial activity of Lactobacillus rhamnosus IMC 501(®) , Lactobacillus paracasei IMC 502(®) and SYNBIO(®) against pathogens. J Appl Microbiol. 2014; 117(2):518-27. DOI: 10.1111/jam.12544. View

Tan Y, Leonhard M, Moser D, Ma S, Schneider-Stickler B . Inhibitory effect of probiotic lactobacilli supernatants on single and mixed non-albicans Candida species biofilm. Arch Oral Biol. 2017; 85:40-45. DOI: 10.1016/j.archoralbio.2017.10.002. View

10.

Hosain Pour A, Salari S, Almani P . Oropharyngeal candidiasis in HIV/AIDS patients and non-HIV subjects in the Southeast of Iran. Curr Med Mycol. 2019; 4(4):1-6. PMC: 6386505. DOI: 10.18502/cmm.4.4.379. View

11.

Mendonca F, Santos S, Faria I, Goncalves E Silva C, Jorge A, Leao M . Effects of probiotic bacteria on Candida presence and IgA anti-Candida in the oral cavity of elderly. Braz Dent J. 2013; 23(5):534-8. DOI: 10.1590/s0103-64402012000500011. View

12.

Kheradmand E, Rafii F, Yazdi M, Sepahi A, Shahverdi A, Oveisi M . The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans. Daru. 2014; 22:48. PMC: 4060857. DOI: 10.1186/2008-2231-22-48. View

13.

Bermudez-Brito M, Plaza-Diaz J, Munoz-Quezada S, Gomez-Llorente C, Gil A . Probiotic mechanisms of action. Ann Nutr Metab. 2012; 61(2):160-74. DOI: 10.1159/000342079. View

14.

Spinler J, Taweechotipatr M, Rognerud C, Ou C, Tumwasorn S, Versalovic J . Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens. Anaerobe. 2008; 14(3):166-71. PMC: 2494590. DOI: 10.1016/j.anaerobe.2008.02.001. View

15.

Strus M, Kucharska A, Kukla G, Brzychczy-Wloch M, Maresz K, Heczko P . The in vitro activity of vaginal Lactobacillus with probiotic properties against Candida. Infect Dis Obstet Gynecol. 2005; 13(2):69-75. PMC: 1784560. DOI: 10.1080/10647440400028136. View

16.

Jiang Q, Stamatova I, Kari K, Meurman J . Inhibitory activity in vitro of probiotic lactobacilli against oral Candida under different fermentation conditions. Benef Microbes. 2014; 6(3):361-8. DOI: 10.3920/BM2014.0054. View

17.

Gil N, Martinez R, Gomes B, Nomizo A, De Martinis E . Vaginal lactobacilli as potential probiotics against Candida SPP. Braz J Microbiol. 2013; 41(1):6-14. PMC: 3768620. DOI: 10.1590/S1517-83822010000100002. View

18.

Bulgasem B, Lani M, Hassan Z, Yusoff W, Fnaish S . Antifungal Activity of Lactic Acid Bacteria Strains Isolated from Natural Honey against Pathogenic Species. Mycobiology. 2017; 44(4):302-309. PMC: 5287163. DOI: 10.5941/MYCO.2016.44.4.302. View

19.

Kaur R, Dhakad M, Goyal R, Bhalla P, Dewan R . Spectrum of Opportunistic Fungal Infections in HIV/AIDS Patients in Tertiary Care Hospital in India. Can J Infect Dis Med Microbiol. 2016; 2016:2373424. PMC: 4931070. DOI: 10.1155/2016/2373424. View

20.

Vilela S, Barbosa J, Rossoni R, Santos J, Prata M, Anbinder A . Lactobacillus acidophilus ATCC 4356 inhibits biofilm formation by C. albicans and attenuates the experimental candidiasis in Galleria mellonella. Virulence. 2015; 6(1):29-39. PMC: 4603435. DOI: 10.4161/21505594.2014.981486. View