Generation of an ESRG Pr-tdTomato Reporter Human Embryonic Stem Cell Line, CSUe011-A, Using CRISPR/Cas9 Editing

ESRG was first identified in our previous study. It is highly expressed in human embryonic stem cells (hESCs), whereas it is significantly down-regulated in differentiated cells and is undetectable in adult tissues. To develop an hESC line for monitoring the expression of ESRG for further study of its function, we used gene editing techniques to insert fusion sequences of ESRG promoter and tdTomato fluorescent protein gene into the AAVS1 human safe harbor locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiation potential.