Self-luminescent Photodynamic Therapy Using Breast Cancer Targeted Proteins

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2020 Sep 12

PMID 32917700

Citations 19

Authors

Eun Hye Kim

Sangwoo Park

Yun Kyu Kim

Minwoo Moon

Jeongwon Park

Kyung Jin Lee

Seongsoo Lee

Young-Pil Kim

Affiliations

Soon will be listed here.

Abstract

Despite the potential of photodynamic therapy (PDT), its comprehensive use in cancer treatment has not been achieved because of the nondegradable risks of photosensitizing drugs and limits of light penetration and instrumentation. Here, we present bioluminescence (BL)-induced proteinaceous PDT (BLiP-PDT), through the combination of luciferase and a reactive oxygen species (ROS)-generating protein (Luc-RGP), which is self-luminescent and degradable. After exposure to coelenterazine- as a substrate for luciferase without external light irradiation, Luc-RGP fused with a small lead peptide-induced breast cancer cell death through the generation of BL-sensitive ROS in the plasma membrane. Even with extremely low light energy, BLiP-PDT exhibited targeted effects in primary breast cancer cells from patients and in in vivo tumor xenograft mouse models. These findings suggest that BLiP-PDT is immediately useful as a promising theranostic approach against various cancers.

Citing Articles

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