Protective Effects of Mycelium on the Development of Osteoarthritis After Monosodium Iodoacetate Injection

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2020 Sep 10

PMID 32908566

Citations 7

Authors

Mi-Rae Shin

Jin A Lee

Min Ju Kim

Hae-Jin Park

Byeong Wook Park

Seung Bo Seo

Seong-Soo Roh

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to identify the protective effects of mycelium (PLM) and its possible mechanisms in a model of monosodium iodoacetate- (MIA-) induced osteoarthritis (OA).

Methods: Intra-articular injection of MIA was injected to 50 L with 80 mg/mL using a 0.3 mL insulin syringe into the right knee joint. Changes in hindpaw weight-bearing distribution between the right (osteoarthritic) and left (contralateral control) legs were used as an index of joint discomfort. PLM (50, 100, and 200 mg/kg body weight) was orally administered once daily for 14 days from day 7 after MIA treatment. And then, various factors associated with inflammatory response and cartilage degeneration in cartilage tissues detected by western blotting.

Results: PLM treatment showed a concentration-dependent elevation in change in hindpaw weight-bearing distribution (HWBD). PLM200 demonstrated the capacity to significantly increase HWBD, indicating that the change in weight-bearing distribution means the reduction of spontaneous pain. Our results indicate that PLM suppressed the inflammatory factors via NF-B signaling pathway induced by p38 phosporlyation. Moreover, PLM200 exhibited a significant reduction of ROS produced by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. PLM100 and PLM200 inhibited the levels of matrix metalloproteinase (MMP)-1, one of proteinase that degrades extracellular matrix (ECM).

Conclusions: Taken together, our results indicated that PLM has a strong chondroprotective effect through the suppression both ROS production and inflammation.

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