Analysis of the Immunomodulating Effects of on Lymphocytes, Macrophages, and Tumour Cells

Overview

Journal J Poult Sci

Date 2020 Sep 10

PMID 32908419

Citations 6

Authors

Youngsub Lee

Sung Hyen Lee

Mi Sun Jeong

Jung Bong Kim

Hwan Hee Jang

Jeong-Sook Choe

Dong Wook Kim

Hyun S Lillehoj

Affiliations

Soon will be listed here.

Abstract

The present study investigated the effects of ethanol extracts of (leaf, root, and fermented root) on parameters of innate immunity, tumour cell viability and antioxidant effect . Innate immunity was measured by spleen lymphocyte proliferation, nitric oxide production by chicken macrophage HD11 cells and suppressive effect on tumour cell viability was assessed using chicken RP9 cells. Free radical scavenging capacity as a measure of antioxidant capacity was determined by 0.15 mM of DPPH solution. culture of chicken spleen lymphocytes with ethanol extract of (62.5-500 g/mL) significantly induced higher proliferation compared with media control. Stimulation of macrophages with ethanol extract of (62.5-500 g/mL) showed increased Nitric oxide production. Tumor cells growth was significantly inhibited by extracts of at 15.6-125 g/mL compared with medium control and all extracts exhibited greater than 80% scavenging activity at 1000 g/mL compared with ethanol vehicle control. Above all, fermented root extracts showed strongest effects on antioxidant activity compared to leaf and root extracts.

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References

Lillehoj H, Lee K . Immune modulation of innate immunity as alternatives-to-antibiotics strategies to mitigate the use of drugs in poultry production. Poult Sci. 2012; 91(6):1286-91. DOI: 10.3382/ps.2012-02374. View

Napoli C, Paolisso G, Casamassimi A, Al-Omran M, Barbieri M, Sommese L . Effects of nitric oxide on cell proliferation: novel insights. J Am Coll Cardiol. 2013; 62(2):89-95. DOI: 10.1016/j.jacc.2013.03.070. View

Oh Y, Cho W, Oh J, Im G, Jeong Y, Yang M . Fermentation by Lactobacillus enhances anti-inflammatory effect of Oyaksungisan on LPS-stimulated RAW 264.7 mouse macrophage cells. BMC Complement Altern Med. 2012; 12:17. PMC: 3323419. DOI: 10.1186/1472-6882-12-17. View

Schubert S, Lansky E, Neeman I . Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. J Ethnopharmacol. 1999; 66(1):11-7. DOI: 10.1016/s0378-8741(98)00222-0. View

Elhelu M . The role of macrophages in immunology. J Natl Med Assoc. 1983; 75(3):314-7. PMC: 2561478. View

Kim D, Lillehoj H, Lee S, Jang S, Park M, Min W . Immune effects of dietary anethole on Eimeria acervulina infection. Poult Sci. 2013; 92(10):2625-34. DOI: 10.3382/ps.2013-03092. View

Meng F, Lowell C . Lipopolysaccharide (LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinases Hck, Fgr, and Lyn. J Exp Med. 1997; 185(9):1661-70. PMC: 2196288. DOI: 10.1084/jem.185.9.1661. View

Lee S, Lillehoj H, Chun H, Tuo W, Park H, Cho S . In vitro treatment of chicken peripheral blood lymphocytes, macrophages, and tumor cells with extracts of Korean medicinal plants. Nutr Res. 2020; 27(6):362-366. DOI: 10.1016/j.nutres.2007.04.001. View

Lillehoj H, Li G . Nitric oxide production by macrophages stimulated with Coccidia sporozoites, lipopolysaccharide, or interferon-gamma, and its dynamic changes in SC and TK strains of chickens infected with Eimeria tenella. Avian Dis. 2004; 48(2):244-53. DOI: 10.1637/7054. View

10.

Hernandez F, Madrid J, Garcia V, Orengo J, Megias M . Influence of two plant extracts on broilers performance, digestibility, and digestive organ size. Poult Sci. 2004; 83(2):169-74. DOI: 10.1093/ps/83.2.169. View

11.

Hong Y, Lillehoj H, Dalloul R, Min W, Miska K, Tuo W . Molecular cloning and characterization of chicken NK-lysin. Vet Immunol Immunopathol. 2006; 110(3-4):339-47. DOI: 10.1016/j.vetimm.2005.11.002. View

12.

Nicotera P, Bonfoco E, Brune B . Mechanisms for nitric oxide-induced cell death: involvement of apoptosis. Adv Neuroimmunol. 1995; 5(4):411-20. DOI: 10.1016/0960-5428(95)00025-9. View

13.

Xu S, Lee S, Lillehoj H, Hong Y, Bravo D . Effects of dietary selenium on host response to necrotic enteritis in young broilers. Res Vet Sci. 2015; 98:66-73. DOI: 10.1016/j.rvsc.2014.12.004. View

14.

Nathan C, Xie Q . Nitric oxide synthases: roles, tolls, and controls. Cell. 1994; 78(6):915-8. DOI: 10.1016/0092-8674(94)90266-6. View

15.

Kaspers B, Lillehoj H, Lillehoj E . Chicken macrophages and thrombocytes share a common cell surface antigen defined by a monoclonal antibody. Vet Immunol Immunopathol. 1993; 36(4):333-46. DOI: 10.1016/0165-2427(93)90029-4. View

16.

Lee S, Lillehoj H, Jang S, Lee K, Bravo D, Lillehoj E . Effects of dietary supplementation with phytonutrients on vaccine-stimulated immunity against infection with Eimeria tenella. Vet Parasitol. 2011; 181(2-4):97-105. DOI: 10.1016/j.vetpar.2011.05.003. View

17.

Kim J, Lillehoj H, Hong Y, Kim G, Lee S, Lillehoj E . Dietary Capsicum and Curcuma longa oleoresins increase intestinal microbiome and necrotic enteritis in three commercial broiler breeds. Res Vet Sci. 2015; 102:150-8. DOI: 10.1016/j.rvsc.2015.07.022. View

18.

Mueller K, Blum N, Kluge H, Mueller A . Influence of broccoli extract and various essential oils on performance and expression of xenobiotic- and antioxidant enzymes in broiler chickens. Br J Nutr. 2011; 108(4):588-602. DOI: 10.1017/S0007114511005873. View

19.

Van Lunen T . Growth performance of pigs fed diets with and without tylosin phosphate supplementation and reared in a biosecure all-in all-out housing system. Can Vet J. 2003; 44(7):571-6. PMC: 340208. View

20.

Vazquez-Prieto M, Miatello R . Organosulfur compounds and cardiovascular disease. Mol Aspects Med. 2010; 31(6):540-5. DOI: 10.1016/j.mam.2010.09.009. View