ITRAQ-Based Proteomics Reveals Potential Anti-Virulence Targets for ESBL-Producing

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2020 Sep 9

PMID 32903891

Citations 5

Authors

Yan Wang

Shan Cong

Qinghua Zhang

Ranwei Li

Ke Wang

Affiliations

Soon will be listed here.

Abstract

Purpose: Treatment of infections with strains producing extended-spectrum beta-lactamases (ESBLs) is challenging due to the coexistence of multiple resistance mechanisms and the hypervirulent variant. Therefore, new targets or more effective treatment options aimed at ESBL-producing are urgently needed.

Materials And Methods: Here, we collected ESBL-producing and non-ESBL isolates and studied their differences from a proteomic point of view.

Results: We revealed treA, wza, gnd, rmlA, rmlC, rmlD, galE, aceE, and sucD as important virulence-related proteins in ESBL-producing , distinct from those in non-ESBL strains.

Conclusion: Our findings provide plausible anti-virulence targets and suggest new therapeutic avenues against ESBL-producing .

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References

Schiraldi C, Di Lernia I, De Rosa M . Trehalose production: exploiting novel approaches. Trends Biotechnol. 2002; 20(10):420-5. DOI: 10.1016/s0167-7799(02)02041-3. View

Verma A, Bhani D, Tomar V, Bachhiwal R, Yadav S . Differences in Bacterial Colonization and Biofilm Formation Property of Uropathogens between the Two most Commonly used Indwelling Urinary Catheters. J Clin Diagn Res. 2016; 10(6):PC01-3. PMC: 4963701. DOI: 10.7860/JCDR/2016/20486.7939. View

Sahly H, Aucken H, Benedi V, Forestier C, Fussing V, Hansen D . Increased serum resistance in Klebsiella pneumoniae strains producing extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 2004; 48(9):3477-82. PMC: 514775. DOI: 10.1128/AAC.48.9.3477-3482.2004. View

Csiszovszki Z, Krishna S, Orosz L, Adhya S, Semsey S . Structure and function of the D-galactose network in enterobacteria. mBio. 2011; 2(4):e00053-11. PMC: 3119520. DOI: 10.1128/mBio.00053-11. View

Chen J, Siu L, Fung C, Lin J, Yeh K, Chen T . Contribution of outer membrane protein K36 to antimicrobial resistance and virulence in Klebsiella pneumoniae. J Antimicrob Chemother. 2010; 65(5):986-90. DOI: 10.1093/jac/dkq056. View

Lee S, Trostel A, Le P, Harinarayanan R, FitzGerald P, Adhya S . Cellular stress created by intermediary metabolite imbalances. Proc Natl Acad Sci U S A. 2009; 106(46):19515-20. PMC: 2780772. DOI: 10.1073/pnas.0910586106. View

Tseng C, Wu H, Wu T, Lin Y, Fung C . Clinical characteristics and outcome of patients with community-onset Klebsiella pneumoniae bacteremia requiring intensive care. J Microbiol Immunol Infect. 2012; 46(3):217-23. DOI: 10.1016/j.jmii.2012.06.001. View

Lawlor M, Handley S, Miller V . Comparison of the host responses to wild-type and cpsB mutant Klebsiella pneumoniae infections. Infect Immun. 2006; 74(9):5402-7. PMC: 1594822. DOI: 10.1128/IAI.00244-06. View

Cantas L, Shah S, Cavaco L, Manaia C, Walsh F, Popowska M . A brief multi-disciplinary review on antimicrobial resistance in medicine and its linkage to the global environmental microbiota. Front Microbiol. 2013; 4:96. PMC: 3653125. DOI: 10.3389/fmicb.2013.00096. View

10.

Arakawa Y, Wacharotayankun R, Nagatsuka T, Ito H, Kato N, Ohta M . Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol. 1995; 177(7):1788-96. PMC: 176807. DOI: 10.1128/jb.177.7.1788-1796.1995. View

11.

Shu H, Fung C, Liu Y, Wu K, Chen Y, Li L . Genetic diversity of capsular polysaccharide biosynthesis in Klebsiella pneumoniae clinical isolates. Microbiology (Reading). 2009; 155(Pt 12):4170-4183. DOI: 10.1099/mic.0.029017-0. View

12.

Paczosa M, Mecsas J . Klebsiella pneumoniae: Going on the Offense with a Strong Defense. Microbiol Mol Biol Rev. 2016; 80(3):629-61. PMC: 4981674. DOI: 10.1128/MMBR.00078-15. View

13.

Shin S, Bae I, Kim J, Jeong S, Yong D, Kim J . Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36. J Med Microbiol. 2011; 61(Pt 2):239-245. DOI: 10.1099/jmm.0.037036-0. View

14.

Roilides E, Simitsopoulou M, Katragkou A, Walsh T . How Biofilms Evade Host Defenses. Microbiol Spectr. 2015; 3(3). DOI: 10.1128/microbiolspec.MB-0012-2014. View

15.

Wu H, Wang A, Jennings M . Discovery of virulence factors of pathogenic bacteria. Curr Opin Chem Biol. 2008; 12(1):93-101. DOI: 10.1016/j.cbpa.2008.01.023. View

16.

Wu M, Lin T, Hsieh P, Yang H, Wang J . Isolation of genes involved in biofilm formation of a Klebsiella pneumoniae strain causing pyogenic liver abscess. PLoS One. 2011; 6(8):e23500. PMC: 3155550. DOI: 10.1371/journal.pone.0023500. View

17.

Niou Y, Wu W, Lin L, Yu M, Shu H, Yang H . Role of galE on biofilm formation by Thermus spp. Biochem Biophys Res Commun. 2009; 390(2):313-8. DOI: 10.1016/j.bbrc.2009.09.120. View

18.

Hamzaoui Z, Ocampo-Sosa A, Fernandez Martinez M, Landolsi S, Ferjani S, Maamar E . Role of association of OmpK35 and OmpK36 alteration and bla and/or bla genes in conferring carbapenem resistance among non-carbapenemase-producing Klebsiella pneumoniae. Int J Antimicrob Agents. 2018; 52(6):898-905. DOI: 10.1016/j.ijantimicag.2018.03.020. View

19.

Kong Q, Beanan J, Olson R, MacDonald U, Shon A, Metzger D . Biofilm formed by a hypervirulent (hypermucoviscous) variant of Klebsiella pneumoniae does not enhance serum resistance or survival in an in vivo abscess model. Virulence. 2012; 3(3):309-18. PMC: 3442843. DOI: 10.4161/viru.20383. View

20.

Vanaporn M, Sarkar-Tyson M, Kovacs-Simon A, Ireland P, Pumirat P, Korbsrisate S . Trehalase plays a role in macrophage colonization and virulence of Burkholderia pseudomallei in insect and mammalian hosts. Virulence. 2016; 8(1):30-40. PMC: 5963195. DOI: 10.1080/21505594.2016.1199316. View