Interferon-γ and IL-10 Release Assay for Patients with Ocular Toxoplasmosis

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2020 Sep 9

PMID 32901605

Authors

Marcelo Rudzinski

Lais Pardini

Mariana Bernstein

Gaston More

Marina Khoury

Silvana Carolina Duarte

Carina Arguelles

Silvia Reina

Jose Raul Oubina

Affiliations

Soon will be listed here.

Abstract

Peripheral blood mononuclear cells (PBMC) from patients with ocular toxoplasmosis were challenged with total antigens from lysate (TATL) in a cytokine release assay (CRA), run during the inactive period of the disease. Increased interferon gamma (IFN-γ) levels were detected after PBMC stimulation with either ME49 reference strain ( = 0.0015) or local TgCkAr-11-9 isolate ( = 0.0012), as compared with those recorded under basal conditions. TATL from TgCkAr11-9 isolate induced a higher release of IFN-γ than ME49 strain in CRA from all tested patients ( = 0.02). The median value of IFN-γ release on TgCkAr-11-9 stimulation (26.03 pg/mL) allowed the classification of patients into high- or low-/non-IFN-γ releasers. Clinical correlations were established with both groups. The results obtained in this study suggest the need to include local strains when performing CRA with TATL.

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