Effect of Api-Bioxal and ApiHerb Treatments Against Infection in Investigated by Two QPCR Methods

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2020 Sep 9

PMID 32899611

Citations 26

Authors

Giovanni Cilia

Claudia Garrido

Martina Bonetto

Donato Tesoriero

Antonio Nanetti

Affiliations

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Abstract

is a worldwide distributed midgut parasite of western honey bees, leading to dwindling colonies and their collapse. As a treatment, only fumagillin is available, causing issues like resistance and hampered bee physiology. This study aimed to evaluate ApiHerb and Api-Bioxal as treatments against . The efficacy was tested using two qPCR methods based on the and genes. In addition, these methods were compared for their aptitude for the quantification of the infection. For this, 19 colonies were selected based on the presence of infections. The colonies were divided into three groups: treated with ApiHerb, Api-Bioxal with previous queen caging and an untreated control. All colonies were sampled pre- and post-treatment. The bees were analyzed individually and in duplicate with both qPCR methods. All bees in the pre-treatment tested positive for . Both treatments reduced the abundance of , but ApiHerb also decreased the prevalence of infected bees. Analysis with the method resulted in several orders of magnitude more copies than analysis with the method. We conclude that both products are suitable candidates for treatment. From our analysis, the qPCR method based on the gene results as more apt for the exact quantification of as is needed for the development of veterinary medicinal products.

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