Analysis in Serum-free Culture of the Targets of Recombinant Human Hemopoietic Growth Factors: Interleukin 3 and Granulocyte/macrophage-colony-stimulating Factor Are Specific for Early Developmental Stages

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1988 Jun 1

PMID 3288992

Citations 27

Authors

Y Sonoda

Y C Yang

G G Wong

S C Clark

M Ogawa

Affiliations

Soon will be listed here.

Abstract

We have used a serum-free culture system for enriched human hemopoietic progenitors to analyze the developmental stages and lineage specificities of the human hemopoietic colony-stimulating factors. None of the individual factors alone efficiently supported hemopoietic colony formation. Neither interleukin 3 nor granulocyte/macrophage-colony-stimulating factor alone or in combination effectively supported proliferation of progenitor cells. However, when combined with granulocyte-colony-stimulating factor or erythropoietin, these factors yielded neutrophil colonies or erythroid bursts, respectively. Serial observations of interleukin 3-supported cultures revealed sequential emergence and subsequent degeneration of clusters of cells. These observations suggest that the primary targets of interleukin 3 and granulocyte/macrophage-colony-stimulating factor are multipotent progenitors at the early stages of development rather than cells in the terminal process of maturation.

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