Splicing Factor SRSF1 Is Essential for Satellite Cell Proliferation and Postnatal Maturation of Neuromuscular Junctions in Mice

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2020 Sep 5

PMID 32888503

Citations 12

Authors

Yuguo Liu

Yangjun Luo

Lei Shen

Ruochen Guo

Zheng Zhan

Ningyang Yuan

Rula Sha

Wenju Qian

Zhenzhen Wang

Zhiqin Xie

Wenwu Wu

Ying Feng

Affiliations

Soon will be listed here.

Abstract

Satellite cells are main muscle stem cells that could provide myonuclei for myofiber growth and synaptic-specific gene expression during the early postnatal development. Here, we observed that splicing factor SRSF1 is highly expressed in myoblasts and its expression is closely related with satellite cell activation and proliferation. By genetic deletion of SRSF1 in myogenic progenitors, we found that SRSF1 is critical for satellite cell proliferation in vitro and in vivo. Most notably we also observed that SRSF1 is required for the functional neuromuscular junction (NMJ) formation, as SRSF1-deficient mice fail to form mature pretzel-like NMJs, which leads to muscle weakness and premature death in mice. Finally, we demonstrated that SRSF1 contributes to muscle innervation and muscle development likely by regulating a restricted set of tissue-specific alternative splicing events. Thus, our data define a unique role for SRSF1 in postnatal skeletal muscle growth and function in mice.

Citing Articles

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Epigenetic control of skeletal muscle atrophy.

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SRSF1 Is Required for Mitochondrial Homeostasis and Thermogenic Function in Brown Adipocytes Through its Control of Ndufs3 Splicing.

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SRSF1-mediated alternative splicing is required for spermatogenesis.

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SRSF10 regulates proliferation of neural progenitor cells and affects neurogenesis in developing mouse neocortex.

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