In-vitro Anticancer Activity Against Caco-2 Cell Line of Colloidal Nano Silver Synthesized Using Aqueous Extract of Leaves

Overview

Journal Heliyon

Specialty Social Sciences

Date 2020 Sep 5

PMID 32885066

Citations 16

Authors

R Zein

I Alghoraibi

Ch Soukkarieh

A Salman

A Alahmad

Affiliations

Soon will be listed here.

Abstract

In the current study, we investigated the anticancer potential against human colon cells (Caco-2) of colloidal nanosilver (CN-Ag) produced in Syria using bioactive compounds in the aqueous extract of leaves (AEECL). The formation of AgNPs was confirmed by UV-visible spectroscopy analysis with surface plasmon peak at 449 nm and their average size was found to be 12, 10, 23 nm by SEM, DLS and NTA respectively. This small size has confirmed the effective role of AEECL as capping agent. Further morphological characterization was done by EDS showed the presence of metallic silver. Zeta potential value (-23 mV) indicated the repulsion among the particles and stability of the formulation nanosilver. The anticancer effect of synthesized CN-Ag against Caco-2 has been tested. The cytotoxicity assay showed a dose-dependent and a time-dependent effect of CN-Ag. The high cytotoxicity of CN-Ag at low concentration (5μ/mL) open new prospects for the development of novel therapeutic approaches against human colon cancer Caco-2.

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