Cell-based Assays to Identify Novel Retinoprotective Agents

Overview

Journal MethodsX

Specialty Pathology

Date 2020 Sep 3

PMID 32874942

Citations 2

Authors

Jeanee Bullock

Glorivee Pagan-Mercado

S Patricia Becerra

Affiliations

Soon will be listed here.

Abstract

Degeneration of the retina can lead ultimately to devastating irreversible vision loss, such as in inherited retinitis pigmentosa and age-related macular degeneration. Currently there is no cure to prevent retinal degeneration. Quantitative cell-based assays can be used to test potential drugs that prevent the death of retinal cells Here, we describe in detail three semi-automated cell-based protocols to identify retinoprotective factors with two retinal cell lines, rat R28 cells and mouse 661W cells. In these protocols, cells are induced to undergo death by photo-oxidation stress, growth factor depletion or cytotoxicity with sodium iodate. Pigment epithelium-derived factor, an established neurotrophic factor for retinal cells, was used as a positive control We discuss how these protocols will prove useful in high-throughput quantitative screening to identify novel therapeutics for retinal disorders.

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