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Association of the Duration of Post-thaw Culture with Clinical Outcome After Vitrified-warmed Day 3 Embryo Transfer in 10,464 Cycles: A Retrospective Cohort Study

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Specialty General Medicine
Date 2020 Sep 3
PMID 32872029
Citations 3
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Abstract

This study aimed to investigate the effect of the duration of embryo culture on clinical outcome in vitrified-warmed cycles.This retrospective cohort study enrolled 10,464 infertile patients, with a total of 18,843 vitrified-warmed day 3 embryos from 2012 to 2017 at a single center. The patients were divided into 2 groups: 9470 cycles in the short-term culture group (0.5-8 hours of post-thaw culture) and 994 cycles in the 48 to 72 hours culture group. The independent effect of the following variables on clinical outcomes was determined: duration of post-thaw culture, maternal age, transferred embryos, embryo quality, and endometrial thickness.We found that the pregnancy rate was positively associated with the post-thaw culture time. Ordinary least square regression analyses showed that the duration of post-thaw culture was positively associated with implantation and live birth rates overall. However, the implantation and live birth rates were not significantly associated with the post-thaw culture time in the short-term culture group. Additionally, maternal age and the number of transferred embryos were independent predictors of the implantation and live birth rates. Moreover, the duration of post-thaw culture did not affect live birth weight.These results indicated that the pregnancy rate is positively associated with the duration of post-thaw culture. Therefore, under the condition of not affecting work shifts, properly prolonging the duration of post-thaw culture to improve the outcome of frozen-thawed embryo transfer should be considered.

Citing Articles

Effect of time post warming to embryo transfer on human blastocyst metabolism and pregnancy outcome.

Ardestani G, Martins M, Ocali O, Sanchez T, Gulliford C, Barrett C J Assist Reprod Genet. 2024; 41(6):1539-1547.

PMID: 38642271 PMC: 11224190. DOI: 10.1007/s10815-024-03115-8.


Effect of post-warming culture time on the live birth rate after frozen embryo transfer.

Pham H, Vu T, Nguyen C, Le A, Nguyen D, Pham T Reprod Med Biol. 2022; 21(1):e12465.

PMID: 35662890 PMC: 9150497. DOI: 10.1002/rmb2.12465.


Does Post-Warming Extended Culture Duration Affect the Clinical and Obstetric Outcomes of Patients of Advanced Maternal Age? A Single-Center Study.

Park J, Ahn S, Seok S, Chang E, Kim J, Kwak I J Korean Med Sci. 2022; 37(12):e96.

PMID: 35347904 PMC: 8960938. DOI: 10.3346/jkms.2022.37.e96.

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