Evaluation of Supercritical CO-Assisted Protocols in a Model of Ovine Aortic Root Decellularization

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2020 Sep 2

PMID 32867356

Citations 4

Authors

Elvira R Gafarova

Ekaterina A Grebenik

Alexey E Lazhko

Anastasia A Frolova

Anastasia S Kuryanova

Alexandr V Kurkov

Ilya A Bazhanov

Byron S Kapomba

Nastasia V Kosheleva

Ivan A Novikov

Anatoly B Shekhter

Elena N Golubeva

Anna B Soloviova

Peter S Timashev

Affiliations

Soon will be listed here.

Abstract

One of the leading trends in the modern tissue engineering is the development of new effective methods of decellularization aimed at the removal of cellular components from a donor tissue, reducing its immunogenicity and the risk of rejection. Supercritical CO (scCO)-assisted processing has been proposed to improve the outcome of decellularization, reduce contamination and time costs. The resulting products can serve as personalized tools for tissue-engineering therapy of various somatic pathologies. However, the decellularization of heterogeneous 3D structures, such as the aortic root, requires optimization of the parameters, including preconditioning medium composition, the type of co-solvent, values of pressure and temperature inside the scCO reactor, etc. In our work, using an ovine aortic root model, we performed a comparative analysis of the effectiveness of decellularization approaches based on various combinations of these parameters. The protocols were based on the combinations of treatments in alkaline, ethanol or detergent solutions with scCO-assisted processing at different modes. Histological analysis demonstrated favorable effects of the preconditioning in a detergent solution. Following processing in scCO medium provided a high decellularization degree, reduced cytotoxicity, and increased ultimate tensile strength and Young's modulus of the aortic valve leaflets, while the integrity of the extracellular matrix was preserved.

Citing Articles

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