Focal Accumulation of ROS Can Block Effector BAS4-Expression and Prevent Infection in Rice

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2020 Sep 2

PMID 32867341

Citations 8

Authors

Yafei Chen

Sarmina Dangol

Juan Wang

Nam-Soo Jwa

Affiliations

Soon will be listed here.

Abstract

The reactive oxygen species (ROS) burst is the most common plant immunity mechanism to prevent pathogen infection, although the exact role of ROS in plant immunity has not been fully elucidated. We investigated the expression and translocation of respiratory burst oxidase homologue B (OsRBOHB) during compatible and incompatible interactions between rice epidermal cells and the pathogenic fungus (syn. ). We characterized the functional role of ROS focal accumulation around invading hyphae during infection process using the OsRBOHB inhibitor diphenyleneiodonium (DPI) and the actin filament polymerization inhibitor cytochalasin (Cyt) A. OsRBOHB was strongly induced during incompatible rice- interactions, and newly synthesized OsRBOHB was focally distributed at infection sites. High concentrations of ROS focally accumulated at the infection sites and suppressed effector biotrophy-associated secreted (BAS) proteins BAS4 expression and invasive hyphal growth. DPI and Cyt A abolished ROS focal accumulation and restored effector BAS4 expression. These results suggest that ROS focal accumulation is able to function as an effective immune mechanism that blocks some effectors including BAS4-expression during infection. Disruption of ROS focal accumulation around invading hyphae enables successful colonization of rice cells and disease development.

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