Sequencing, Assembly, Annotation, and Gene Expression: Novel Insights into Browning-resistant

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2020 Sep 1

PMID 32864209

Citations 3

Authors

Ya-Hui Wang

Xiao-Hong Liu

Rong-Rong Zhang

Zhi-Ming Yan

Ai-Sheng Xiong

Xiao-Jun Su

Affiliations

Soon will be listed here.

Abstract

is a kind of melon crop widely cultivated in temperate regions worldwide. Browning is one of the serious factors affecting the quality of . Therefore, the molecular mechanism of browning is of great significance to study. However, the molecular diversity of cultivars with different browning-resistant abilities has not been well elucidated. In our study, we used high-throughput sequencing to determine the transcriptome of two cultivars '2D-2' and '35D-7'. A total of 115,099 unigenes were clustered, of which 22,607 were differentially expression genes (DEGs). Of these DEGs, 65 encoding polyphenol oxidase, peroxidase, or ascorbate peroxidase were further analyzed. The quantitative real-time PCR (RT-qPCR) data indicated that the expression levels of the gene (Accession No.: Cluster-21832.13892) was significantly higher in '35D-7' compared with that in '2D-2'. Several genes (Accession No.: Cluster-21832.19847, Cluster-21832.30619 and Cluster-48491.2) were also upregulated. Analysis of the plantTFDB database indicated that some transcription factors such as WRKY gene family may also participate in the regulation of browning. The results indicated that the divergence of genes expression related to enzymatic reaction may lead to the different browning resistances of . Our study will provide a theoretical basis for breeding of browning-resistant .

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