Intraluminal Infusion of Penta-Galloyl Glucose Reduces Abdominal Aortic Aneurysm Development in the Elastase Rat Model

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2020 Aug 29

PMID 32857766

Citations 10

Authors

Asbjorn Sune Schack

Jane Stubbe

Lasse Bach Steffensen

Hend Mahmoud

Malene Skaarup Laursen

Jes Sanddal Lindholt

Affiliations

Soon will be listed here.

Abstract

Background: An abdominal aortic aneurysm (AAA) is a progressive chronic dilatation of the abdominal aorta with terminally rupture when the aortic wall is so weakened that aortic wall stress exceeds wall strength. No effective medical treatment exists so far. We aimed to test whether intraluminal admission of Penta-Galloyl Glucose (PGG) treatment in a rodent AAA model could hold the potential to inhibit aneurysmal progression.

Method: Male Sprague Dawley rats had either intraluminal elastase infused for AAA induction or saline to serve as controls. In two independent experimental series, elastase was used to induce AAA followed by an intraluminal PGG (directly or by a drug eluting balloon) treatment. All rats were followed for 28 days and euthanized. In both series, maximal infrarenal aortic diameter was measured at baseline and at termination as a measure of AAA size. In series 2, maximal internally AAA diameter was followed by ultrasound weekly. AAA tissues were analyzed for elastin integrity by millers stain, collagen deposition by masson trichrome staining. In other AAA tissue samples the mRNA level of CD45, lysyloxidase (LOX), lysyloxidase like protein 1 (LOXL1) were determined by qPCR.

Results: Direct administration of PGG significantly reduced AAA expansion when compared to controls. PGG treatment resulted in a higher number and more preserved elastic fibers in the aneurysmal wall, while no significant difference was seen in the levels of CD45 and LOX mRNA levels. The drug eluting balloon (DEB) experiment showed no significant difference in AAA size observed neither macroscopically nor ultrasonically. Also the aneurysmal mRNA levels of CD45, LOX and LOXL1 were unchanged between groups.

Conclusion: A significant reduced expansion of AAAs was observed in the PGG group, suggesting PGG as a drug to inhibit aneurysmal progression, while administration through a DEB did not show a promising new way of administration.

Citing Articles

Binding of Pentagalloyl Glucose to Aortic Wall Proteins: Insights from Peptide Mapping and Simulated Docking Studies.

Simionescu D, Tharayil N, Leonard E, Carlyle W, Schwarz A, Ning K Bioengineering (Basel). 2023; 10(8).

PMID: 37627822 PMC: 10451288. DOI: 10.3390/bioengineering10080936.

Novel pharmacological approaches in abdominal aortic aneurysm.

Puertas-Umbert L, Almendra-Pegueros R, Jimenez-Altayo F, Sirvent M, Galan M, Martinez-Gonzalez J Clin Sci (Lond). 2023; 137(15):1167-1194.

PMID: 37559446 PMC: 10415166. DOI: 10.1042/CS20220795.

Dexmedetomidine Alleviates Abdominal Aortic Aneurysm by Activating Autophagy Via AMPK/mTOR Pathway.

Yu Q, Zeng S, Hu R, Li M, Liu Q, Wang Y Cardiovasc Drugs Ther. 2023; 39(1):33-42.

PMID: 37392236 DOI: 10.1007/s10557-023-07483-8.

Pentagalloyl Glucose (PGG) Prevents and Restores Mechanical Changes Caused by Elastic Fiber Fragmentation in the Mouse Ascending Aorta.

Crandall C, Caballero B, Viso M, Vyavahare N, Wagenseil J Ann Biomed Eng. 2022; 51(4):806-819.

PMID: 36203118 PMC: 10117999. DOI: 10.1007/s10439-022-03093-x.

Establishment of a New Abdominal Aortic Aneurysm Model in Rats by a Retroperitoneal Approach.

Zhu J, Tang Q, Zhou C, Shi X, Yi S, Yang Y Front Cardiovasc Med. 2022; 9:808732.

PMID: 35282381 PMC: 8905142. DOI: 10.3389/fcvm.2022.808732.

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