Antimycotic Activity of Ozonized Oil in Liposome Eye Drops Against Spp

Overview

Journal Transl Vis Sci Technol

Specialties Biomedical Engineering
Ophthalmology

Date 2020 Aug 29

PMID 32855851

Citations 14

Authors

Giuseppe Celenza

Roberto Iorio

Salvatore Cracchiolo

Sabrina Petricca

Ciro Costagliola

Benedetta Cinque

Bernardetta Segatore

Gianfranco Amicosante

Pierangelo Bellio

Affiliations

Soon will be listed here.

Abstract

Purpose: This study aims to investigate the antifungal activity and mechanism of action of ozonized oil eye drops in liposomes (Ozodrop), commercialized as eye lubricant for the treatment of dry eye syndrome and eye inflammation. The activity was tested against four clinical species: , and .

Methods: The antifungal activity of the eye drop solution was ascertained by microdilution method in accordance with EUCAST obtaining the minimum inhibitory concentration for Ozodrop. The mechanism of action was further investigated in by measuring cell vitality, intracellular reactive oxygen species production, levels of cellular and mitochondrial (∆Ψ) membrane potential, and the extent of membrane lipid peroxidation.

Results: All isolates were susceptible to Ozodrop with minimum inhibitory concentration values ranging from 0.195% (v/v) for to 6.25% (v/v) for . After 1 hour of exposure at the minimum inhibitory concentration value about 30% of cells were killed, reaching about 70% at the highest Ozodrop value. After Ozodrop exposure, showed cell membrane depolarization, increased levels of lipid peroxidation, depolarized ∆Ψ, and increased reactive oxygen species generation.

Conclusions: The significant increases in reactive oxygen species production cause the accumulation of reactive oxygen species-associated damages leading to progressive Candida cell dysfunction.

Translational Relevance: The antifungal activity of Ozodrop was demonstrated at concentrations several times lower than the concentration that can be retrieved in ocular surface after its application. The antifungal activity of the eye drops Ozodrop would represent an interesting off-label indication for a product basically conceived as an eye lubricant.

Citing Articles

Liposomal Ozonated Oil Ensures a Further Reduction in the Microbial Load Before Intravitreal Injection: the "OPERA" Study.

Grassi M, Boscia G, Alessio G, Zerbinati M, Petrara G, Puzo P Ophthalmol Ther. 2024; 13(10):2771-2788.

PMID: 39222288 PMC: 11408443. DOI: 10.1007/s40123-024-01006-w.

Ozonated Oil in Liposome Eyedrops Reduces the Formation of Biofilm, Selection of Antibiotic-Resistant Bacteria, and Adhesion of Bacteria to Human Corneal Cells.

Gentili V, Strazzabosco G, Salgari N, Mancini A, Rizzo S, Beltrami S Int J Mol Sci. 2023; 24(18).

PMID: 37762377 PMC: 10531138. DOI: 10.3390/ijms241814078.

Evaluation of the efficacy of ozonated olive oil for controlling the growth of and its toxins.

Sehim A, Abd Elghaffar R, Emam A, El-Desoukey T Heliyon. 2023; 9(7):e17885.

PMID: 37483790 PMC: 10359872. DOI: 10.1016/j.heliyon.2023.e17885.

Prophylaxis of Ocular Infection in the Setting of Intraocular Surgery: Implications for Clinical Practice and Risk Management.

Borgia A, Mazzuca D, Della Corte M, Gratteri N, Fossati G, Raimondi R Ophthalmol Ther. 2023; 12(2):721-734.

PMID: 36719608 PMC: 10011360. DOI: 10.1007/s40123-023-00661-9.

Ozonated oil is effective at killing Candida species and Streptococcus mutans biofilm-derived cells under aerobic and microaerobic conditions.

Higa B, Cintra B, Alvarez C, Ribeiro A, Ferreira J, Tavares D Med Mycol. 2022; 60(8).

PMID: 35869980 PMC: 9359064. DOI: 10.1093/mmy/myac055.

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